Print Email Facebook Twitter Activation of toll-like receptors and dendritic cells by a broad range of bacterial molecules Title Activation of toll-like receptors and dendritic cells by a broad range of bacterial molecules Author Boele, L.C.L. Bajramovic, J.J. de Vries, A.M.M.B.C. Voskamp-Visser, I.A.I. Kaman, W.E. van der Kleij, D. TNO Defensie en Veiligheid Publication year 2009 Abstract Activation of pattern recognition receptors such as Toll-like receptors (TLRs) by pathogens leads to activation and maturation of dendritic cells (DC), which orchestrate the development of the adaptive immune response. To create an overview of the effects of a broad range of pathogenic bacteria, their capacity to activate TLRs and to affect DC maturation, cytokine production and T cell polarizing capacity were determined. Different bacterial species differed in their potency to affect these parameters. In general, on the DC level differences were found in the maturation-inducing capacity of gram-negative and gram-positive bacteria. Remarkably, these differences did not result in differential polarization of the T cell response. With respect to TLRs, TLR4 activation by pathogens correlated with their ability to induce DC maturation, while for TLR2 and TLR5 such a correlation was absent. Taken together, this study provides insight into qualitative differences and general effects of pathogen-derived molecules on dendritic cells. © 2008 Elsevier Inc. All rights reserved. Chemicals / CAS: toll like receptor 2, 203811-81-8; toll like receptor 4, 203811-83-0; toll like receptor, 409141-78-2; Antigens, CD; Biological Markers; Cytokines; HLA-DR Antigens; Ligands; Protein Isoforms; Toll-Like Receptors Subject BiologyBacteriaCytokinesDendritic cellPathogensToll-like receptortoll like receptortoll like receptor 2toll like receptor 4toll like receptor 5articlecell maturationcell polaritycontrolled studycytokine productiondendritic cellgenetic transfectionhumanhuman cellpriority journalStaphylococcus aureusT lymphocyteVibrio choleraeYersinia pestisYersinia pseudotuberculosisAnimalsAntigens, CDBacteriaBiological MarkersCell LineCoculture TechniquesCytokinesDendritic CellsHLA-DR AntigensHumansLigandsProtein IsoformsT-LymphocytesToll-Like ReceptorsUltraviolet RaysBacteria (microorganisms)NegibacteriaPosibacteria To reference this document use: http://resolver.tudelft.nl/uuid:0aecd1df-bd23-43ca-b4b1-83a13ec063c3 DOI https://doi.org/10.1016/j.cellimm.2008.09.002 TNO identifier 27880 Source Cellular Immunology, 255 (1-2), 17-25 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.