Title
Complement-resistant Moraxella catarrhalis forms a genetically distinct lineage within the species
Author
Verduin, C.M.
Kools-Sijmons, M.
van der Plas, J.
Vlooswijk, J.
Tromp, M.
van Dijk, H.
Banks, J.
verbrugh, H.
van Belkum, A.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
2000
Abstract
Moraxella catarrhalis is a bacterial species that has been implicated in 15^20% of all cases of otitis media in the USA and the complementresistantvariant of M. catarrhalis has been considered particularly pathogenic. A collection of geographically diverse, complement-sensitive (n = 28) and -resistant strains (n = 47) of M. catarrhalis was assembled in order to analyse the bacterial population structure. All strains were identified as M. catarrhalis by conventional microbiological and biochemical methods. Amplification of the small subunit (ssu) ribosomal RNA gene followed by restriction fragment length polymorphism (RFLP) analysis did not reveal consistent differences between serumsusceptible and -resistant M. catarrhalis isolates. Interestingly, upon automated ribotyping using the Qualicon RiboPrinter0 microbial characterisation system, the complement-sensitive and -resistant strains segregated into two groups. This suggested the existence of two clearly distinguishable lineages within the species M. catarrhalis. This observation was corroborated by pulsed field gel electrophoresis (PFGE) of DNA macro-restriction fragments, a non-ribosomal PCR RFLP procedure and random amplification of polymorphic DNA (RAPD) analysis. All procedures grouped the two variants similarly. Redefinition of the taxonomic status of complement-resistant M. catarrhalis or even the definition of a new species may be opportune. Chemicals/CAS: Complement System Proteins, 9007-36-7; DNA, Bacterial; DNA, Ribosomal
Subject
Automated ribotyping
Complement resistance
Moraxella catarrhalis
Pulsed field gel electrophoresis
Bacterial genetics
Gene amplification
Moraxella catarrhalis
Nonhuman
Otitis media
Polymerase chain reaction
Priority journal
Random amplified polymorphic DNA
Restriction fragment length polymorphism
Bacterial Typing Techniques
Complement System Proteins
DNA, Bacterial
DNA, Ribosomal
Electrophoresis, Gel, Pulsed-Field
Genes, rRNA
Genotype
Moraxella (Branhamella) catarrhalis
Polymorphism, Restriction Fragment Length
Random Amplified Polymorphic DNA Technique
Bacteria (microorganisms)
Moraxella catarrhalis
Negibacteria
Prokaryota
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DOI
https://doi.org/10.1016/s0378-1097(00)00010-0
TNO identifier
86992
ISSN
0378-1097
Source
Fems Microbiology Letters, 184 (1), 1-8
Document type
article