Print Email Facebook Twitter Protein and messenger RNA levels of plasminogen activators and inhibitors analyzed in 22 human tumor cell lines Title Protein and messenger RNA levels of plasminogen activators and inhibitors analyzed in 22 human tumor cell lines Author Quax, P.H.A. van Leeuwen, R.T.J. Verspaget, H.W. Verheijen, J.H. Gaubius Instituut TNO Publication year 1990 Abstract In 22 human tumor cell lines the regulation of production of plasminogen activators urokinase (u-PA) and tissue-type (u-PA) and their inhibitors PAI-1 and PAI-2 was studied. These four components may determine the net plasminogen activator activity, which is often associated with tumor development and metastatic processes. The amount of specific mRNA and protein produced by the cells was measured for all four components. The frequent finding of t-PA (alone or in combination with t-PA) suggests that t-PA can also be a tumor-associated plasminogen activator. In 11 of the 22 cells PAI-1 mRNA and in 6 of the 22 cells PAI-2 mRNA was found, pointing to a possible role of plasminogen activator inhibitors in the tumor-related plasminogen activator activity. This study demonstrates that there are at least two important regulatory steps in the regulation of production of plasminogen activators and their inhibitors: (a) the regulation at the mRNA level, since a high protein amount is always correlated with a high mRNA amount found in the tumor cells; (b) there must be a significant regulatory step at the (post)translational level as can be concluded from differences in mRNA usage. Subject BiologyPlasminogen ActivatorsPlasminogen InactivatorsRNA, MessengerTissue Plasminogen ActivatorTranscription, GeneticTumor Cells, CulturedUrinary Plasminogen Activator To reference this document use: http://resolver.tudelft.nl/uuid:09d41f4e-3499-40ec-a257-a4098abbe588 TNO identifier 231020 ISSN 0008-5472 Source Cancer Research, 50 (5), 1488-1494 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.