Title
The metabolic activity of fecal microbiota from healthy individuals and patients with inflammatory bowel disease
Author
van Nuenen, M.H.M.C.
Venema, K.
van der Woude, J.C.J.
Kuipers, E.J.
TNO Voeding
Publication year
2004
Abstract
The hypothesis was studied that intestinal microbial metabolites play a role in the pathogenesis of inflammatory bowel disease. For that purpose, an in vitro model of the colon was inoculated with fresh feces of six healthy individuals and eight inflammatory bowel disease patients. Samples were taken from the model over time to analyze metabolites from both saccharolytic and proteolytic fermentation. Microbiotas from inflammatory bowel disease patients produced significantly more short-chain fatty acids and ammonia than microbiotas from healthy individuals. Furthermore, the branched-chain fatty acid production was 25% higher after inoculation with microbiotas from patients than after inoculation with microbiotas from healthy individuals. Phenolic compounds were produced by all microbiotas, with large interindividual variation. The production of (potentially toxic) metabolites may play a role in the onset or chronicity of inflammatory bowel disease, because they were produced in higher amounts by microbiotas from these patients than by microbiotas from healthy individuals.
Subject
Nutrition
Physiological Sciences
Fermentation
In vitro model
Inflammatory bowel disease
Metabolites
Microbial activity
ammonia
branched chain fatty acid
fatty acid
lactic acid
phenol derivative
short chain fatty acid
unclassified drug
adult
article
carbohydrate metabolism
chronicity
clinical article
controlled study
enteritis
fatty acid synthesis
feces microflora
fermentation
human
in vitro study
metabolite
microbial metabolism
model
nonhuman
normal human
priority journal
protein degradation
Adult
Ammonia
Bacteria
Colitis, Ulcerative
Colon
Crohn Disease
Fatty Acids
Fatty Acids, Volatile
Feces
Fermentation
Humans
Lactic Acid
Middle Aged
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http://resolver.tudelft.nl/uuid:0954d11f-37fd-40c1-a7c8-9063f2106bba
DOI
https://doi.org/10.1023/b:ddas.0000020508.64440.73
TNO identifier
237660
ISSN
0163-2116
Source
Digestive Diseases and Sciences, 49 (3), 485-491
Document type
article