Print Email Facebook Twitter Lactobacillus casei Shirota does not decrease the food allergic response to peanut extract in Brown Norway rats Title Lactobacillus casei Shirota does not decrease the food allergic response to peanut extract in Brown Norway rats Author de Jonge, J.D. Ezendam, J. Knippels, L.M.J. Penninks, A.H. Pieters, R. van Loveren, H. TNO Kwaliteit van Leven Publication year 2008 Abstract Probiotics are claimed to beneficially affect the immune system and their involvement in allergy prevention is being investigated extensively. However, the efficacy of probiotics in allergy prevention remains controversial. We investigated whether the probiotic Lactobacillus casei Shirota (LcS) could modulate the food allergic response against peanut extract (PE) in Brown Norway (BN) rats. For this purpose BN rats were sensitized to PE (0, 1 and 10 mg/(rat d)) by daily oral gavage and the LcS-groups were additionally orally dosed with 1 × 109 colony forming units LcS/(rat d). LcS administration had minor effects in animals that were not sensitized. LcS increased Th1-(PE-specific IgG1), whereas the Th1/Th2 ratio based on PE-specific IgG1/PE-specific IgG2a shifted towards Th2 dominance in rats sensitized to PE in the presence of LcS as compared to rats that were sensitized to PE only. LcS stimulated PE-specific IgG2a; but for PE-specific IgE the effect was less clear; whereas there was no overall effect, two rats did not show detectable specific IgE antibodies, whereas the remainder showed significantly increased levels. LcS also resulted in increased numbers of basophilic granulocytes in blood. Furthermore, LcS increased levels of both Th1-(IFN-γ) and Th2-(IL-4) related cytokines in PE stimulated spleen and mesenteric lymph node (MLN) cells, but predominantly IL-4 levels in the supernatants of both spleens and MLNs. Our study does not support the hypothesis that LcS down-regulates food allergic responses in a BN rat model for food allergy to peanut. © 2008 Elsevier Ireland Ltd. All rights reserved. Subject NutritionBrown Norway ratFood allergyLactobacillus casei ShirotaPeanutarachis oilgamma interferonimmunoglobulin E antibodyimmunoglobulin G2ainterleukin 4mast cell protease 2probiotic agentserine proteinaseanimal cellanimal experimentanimal modelantibody blood levelantibody titerarticlebacterial strainbasophilbrown Norway ratcontrolled studycytokine productiondrug effectfemaleLactobacillus caseimesentery lymph nodenonhumanpeanut allergypriority journalprovocation testratsensitizationspleen cellTh1 cellTh2 cellAnimalsBasophilsCell CountChymasesCytokinesDigoxigeninEndotoxinsImmunoglobulin EImmunoglobulin GLactobacillus caseiLeukocyte CountPeanut HypersensitivityPlant ExtractsProbioticsRatsRats, Inbred BNTh1 CellsTh2 CellsAnimaliaArachis hypogaeaLactobacillus caseiRattusRattus norvegicus To reference this document use: http://resolver.tudelft.nl/uuid:083fc566-9271-4b72-9dce-3195c00d7fa5 DOI https://doi.org/10.1016/j.tox.2008.04.016 TNO identifier 240921 ISSN 0300-483X Source Toxicology, 249 (2-3), 140-145 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.