Title
Evaluation of monoclonal antibodies with specificity for human IgA, IgA subclasses and allotypes and secretory component. Results of an IUIS/WHO collaborative study
Author
Mestecky, J.
Hamilton, R.G.
Magnusson, C.G.M.
Jefferis, R.
Vaerman, J.P.
Goodall, M.
de Lange, G.G.
Moro, I.
Aucouturier, P.
Radl, J.
Cambiaso, C.
Silvain, C.
Preud'homme, J.L.
Kusama, K.
Carlone, G.M.
Biewenga, J.
Kobayashi, K.
Skvaril, F.
Reimer, C.B.
Gaubius Instituut TNO
Publication year
1996
Abstract
51 monoclonal antibodies (McAb) with putative specificity for human IgA, the IgA subclasses, Am allotypes or secretory component (SC) were evaluated for immunoreactivity and specificity by nine laboratories employing immunodiffusion, agglutination, immunohistological assays, immunoblotting and direct binding and competitive inhibition enzyme immunoassays. McAbs specific for IgA PAN (n = 24), IgA1 (n = 7), IgA2 (n = 3), IgA2m(2) (n = 2), non-IgA2m(2) (n = 4) and SC or secretory IgA (n = 5) were identified that were immunoreactive and specific in the assays employed. The McAbs identified as IgA- or SC-reactive were shown to be non-reactive to human IgG, IgM, IgD, IgE, κ and λ by-direct binding and competitive inhibition immunoassays. Interestingly, no McAbs with restricted specificity for IgA2m(l) were identified. Some McAb displayed higher affinity and/or better performance in one or several of the assay groups. The IgA- and SC-specific McAbs identified in this international collaborative study have potential as immunochemical reference reagents to identify and quantitate monomeric and polymeric IgA in human serum and secretions. Chemicals/CAS: Antibodies, Monoclonal; Binding Sites, Antibody; Epitopes; Immunoglobulin A; Immunoglobulin A, Secretory; Immunoglobulin Allotypes; Secretory Component
Subject
Biology
(Human)
Am allotype
IgA
IgA subclass
Monoclonal antibody
Specificity
immunoglobulin a
immunoglobulin subclass
monoclonal antibody
antigen antibody reaction
article
controlled study
diagnostic value
enzyme immunoassay
human
immunoblotting
immunodiffusion
immunohistology
immunoreactivity
priority journal
serum
Animals
Antibodies, Monoclonal
Antibody Specificity
Binding Sites, Antibody
Epitopes
Humans
Immunoglobulin A
Immunoglobulin A, Secretory
Immunoglobulin Allotypes
Immunologic Techniques
Mice
Reference Standards
Secretory Component
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DOI
https://doi.org/10.1016/0022-1759(95)00289-8
TNO identifier
233366
ISSN
0022-1759
Source
Journal of Immunological Methods, 193 (2), 103-148
Document type
article