Print Email Facebook Twitter Transcriptome and proteome profiling of colon mucosa from quercetin fed F344 rats point to tumor preventive mechanisms, increased mitochondrial fatty acid degradation and decreased glycolysis Title Transcriptome and proteome profiling of colon mucosa from quercetin fed F344 rats point to tumor preventive mechanisms, increased mitochondrial fatty acid degradation and decreased glycolysis Author Dihal, A.A. van der Woude, H. Hendriksen, P.J.M. Charif, H. Dekker, L.J. IJsselstijn, L. de Boer, V.C.J. Alink, G.M. Burgers, P.C. Rietjens, I.M.C.M. Woutersen, R.A. Stierum, R.H. TNO Kwaliteit van Leven KvL Publication year 2008 Abstract Quercetin has been shown to act as an anticarcinogen in experimental colorectal cancer (CRC). The aim of the present study was to characterize transcriptome and proteome changes occurring in the distal colon mucosa of rats supplemented with 10 g quercetin/kg diet for 11 wk. Transcriptome data analyzed with Gene Set Enrichment Analysis showed that quercetin significantly downregulated the potentially oncogenic mitogen-activated protein kinase (Mapk) pathway. In addition, quercetin enhanced expression of tumor suppressor genes, including Pten, Tp53, and Msh2, and of cell cycle inhibitors, including Mutyh. Furthermore, dietary quercetin enhanced genes involved in phase I and II metabolism, including Fmo5, Ephx1, Ephx2, and Gpx2. Quercetin increased PPARα target genes, and concomitantly enhanced expression of genes involved in mitochondrial fatty acid (FA) degradation. Proteomics performed in the same samples revealed 33 affected proteins, of which four glycolysis enzymes and three heat shock proteins were decreased. A proteome-transcriptome comparison showed a low correlation, but both pointed out toward altered energy metabolism. In conclusion, transcriptomics combined with proteomics showed that dietary quercetin evoked changes contrary to those found in colorectal carcinogenesis. These tumor-protective mechanisms were associated with a shift in energy production pathways, pointing at decreased cytoplasmic glycolysis and toward increased mitochondrial FA degradation. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA. Molecular Sequence Numbers: GENBANK: A23126, A24639, A26186, A26468, A35340, A35865, A40452, A54889, AAA18026, AAA40933, AAB23369, AAC52676, AAH62238, AAH64440, AAH78829, AAH87023, AAP13984, BAA28215, CAA65655, I52328, I65237, Q63654, S17189; Chemicals / CAS: mitogen activated protein kinase, 142243-02-5; peroxisome proliferator activated receptor alpha, 147258-70-6; quercetin, 117-39-5; Fatty Acids; Proteome; Quercetin, 117-39-5 Subject BiologyToxicology and Applied PharmacologyBiomarkerColorectal cancerQuercetinTranscriptomicsfatty acidglutathione peroxidase 2heat shock proteinmitogen activated protein kinaseperoxisome proliferator activated receptor alphaquercetintranscriptomeanimal experimentanimal modelarticlecancer preventioncell cyclecolon mucosacolorectal cancercontrolled studydown regulationenergy metabolismFischer 344 ratgene expressiongene targetinggenetic analysisglycolysislipid degradationmalenonhumannucleotide sequencepriority journalproteomicsratstatistical significancetumor biopsyAnimalsColonColorectal NeoplasmsDietDown-RegulationFatty AcidsGene Expression ProfilingGlycolysisIntestinal MucosaMaleMitochondriaProteomeQuercetinRatsRats, Inbred F344Rattus To reference this document use: http://resolver.tudelft.nl/uuid:06ced032-753b-40cf-b5e6-366ff7fa250f DOI https://doi.org/10.1002/pmic.200700364 TNO identifier 240575 ISSN 1615-9853 Source Proteomics, 8 (1), 45-61 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.