Print Email Facebook Twitter The bovine oocyte in vitro maturation model: A potential tool for reproductive toxicology screening Title The bovine oocyte in vitro maturation model: A potential tool for reproductive toxicology screening Author Beker-van Woudenberg, A.C.R.L. Gröllers-Mulderij, M. Snel, C. Jeurissen, N. Stierum, R. Wolterbeek, A. Publication year 2012 Abstract Reproductive toxicity testing according to the present guidelines requires a high number of animals. Therefore, the development of alternative in vitro methods is urgently required. The aim of the present study was to investigate the applicability domain of the bovine oocyte in vitro maturation assay (bIVM) to study female reproductive toxicology. Therefore, bovine oocytes were exposed to a broad set of chemicals of two distinct biological function groups: (a) affecting female fertility and (b) affecting embryonic development and having a broad range of physical and chemical properties. The endpoints evaluated were the oocyte nuclear maturation (progression of meiosis) and general cytotoxicity. The oocyte nuclear maturation was negatively affected by all compounds tested and the effect was observed at concentrations lower than the cytotoxic ones. The bIVM assay correctly predicted the classification of compounds between those predefined groups. Additionally, the bIVM model contributes significantly for the 3R principle, since no test animals are used in this assay. In conclusion, the bIVM is a sensitive and valuable alternative assay to identify potential chemical hazard on female fertility. © 2012 Elsevier Inc. Subject Life Life Triskelion BVMSB - Microbiology and Systems Biology QS - Quality & SafetyEELS - Earth, Environmental and Life SciencesBiomedical InnovationHealthHealthy LivingAlternative testBovineFemale fertilityIn vitro maturationOocyteReproductive toxicology To reference this document use: http://resolver.tudelft.nl/uuid:04c0ad51-5930-4842-835a-826ecc7ebca1 DOI https://doi.org/10.1016/j.reprotox.2012.05.098 TNO identifier 462853 ISSN 0890-6238 Source Reproductive Toxicology, 34 (2), 251-260 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.