Title
The bovine oocyte in vitro maturation model: A potential tool for reproductive toxicology screening
Author
Beker-van Woudenberg, A.C.R.L.
Gröllers-Mulderij, M.
Snel, C.
Jeurissen, N.
Stierum, R.
Wolterbeek, A.
Publication year
2012
Abstract
Reproductive toxicity testing according to the present guidelines requires a high number of animals. Therefore, the development of alternative in vitro methods is urgently required. The aim of the present study was to investigate the applicability domain of the bovine oocyte in vitro maturation assay (bIVM) to study female reproductive toxicology. Therefore, bovine oocytes were exposed to a broad set of chemicals of two distinct biological function groups: (a) affecting female fertility and (b) affecting embryonic development and having a broad range of physical and chemical properties. The endpoints evaluated were the oocyte nuclear maturation (progression of meiosis) and general cytotoxicity. The oocyte nuclear maturation was negatively affected by all compounds tested and the effect was observed at concentrations lower than the cytotoxic ones. The bIVM assay correctly predicted the classification of compounds between those predefined groups. Additionally, the bIVM model contributes significantly for the 3R principle, since no test animals are used in this assay. In conclusion, the bIVM is a sensitive and valuable alternative assay to identify potential chemical hazard on female fertility. © 2012 Elsevier Inc.
Subject
Life Life Triskelion BV
MSB - Microbiology and Systems Biology QS - Quality & Safety
EELS - Earth, Environmental and Life Sciences
Biomedical Innovation
Health
Healthy Living
Alternative test
Bovine
Female fertility
In vitro maturation
Oocyte
Reproductive toxicology
To reference this document use:
http://resolver.tudelft.nl/uuid:04c0ad51-5930-4842-835a-826ecc7ebca1
DOI
https://doi.org/10.1016/j.reprotox.2012.05.098
TNO identifier
462853
ISSN
0890-6238
Source
Reproductive Toxicology, 34 (2), 251-260
Document type
article