Print Email Facebook Twitter Metabolomics (liver and blood profiling) in a mouse model in response to fasting: A study of hepatic steatosis Title Metabolomics (liver and blood profiling) in a mouse model in response to fasting: A study of hepatic steatosis Author van Ginneken, V. Verhey, E. Poelmann, R. Ramakers, R. van Dijk, K.W. Ham, L. Voshol, P. Havekes, L. van Eck, M. van der Greef, J. TNO Kwaliteit van Leven Publication year 2007 Abstract A metabolomic approach was applied to a mouse model of starvation-induced hepatic steatosis. After 24 h of fasting it appears that starvation reduced the phospholipids (PL), free cholesterol (FC), and cholesterol esters (CE) content of low-density lipoproteins (LDL). In liver lipid profiles major changes were observed using different techniques. High performance thin layer chromatography (HPTLC)-measurements of liver-homogenates indicated a significant rise of FC with 192%, triacylglycerols (TG) with 456% and cholesterol esters (CE) with 268% after 24 h of starvation in comparison with the control group. Reversed phase liquid chromatography coupled to mass spectrometry measurements (LC-MS) of liver homogenate indicated that the intensity of Phosphatidylcholine (PC) in the 24-h starvation group dropped to 90% of the value in the control group while the intensity of CE and TG increased to 157% and 331%, respectively, of the control group. Interestingly, a 49:4-TG with an odd number of C atoms appeared during starvation. This unique triacylglycerol has all characteristics of a biomarker for detection of hepatic steatosis. These observations indicate that in mammals liver lipid profiles are a dynamic system which are readily modulated by environmental factors like starvation. © 2007 Elsevier B.V. All rights reserved. Subject Analytical researchBiomedical researchHepatic steatosisLipidsLiverMetabolomicsStarvationTriacylglycerolscholesterolcholesterol esterlow density lipoprotein cholesterolphosphatidylcholinephospholipid derivativetriacylglycerolanimal experimentanimal modelanimal tissuearticleblood examinationcontrolled studydiet restrictionfatty liverhigh performance thin layer chromatographylipid blood levellipid storageliver homogenatemalemass spectrometrymetabolomicsmousenonhumanpriority journalreversed phase liquid chromatographystarvationAnimalsBloodCholesterol EstersChromatography, High Pressure LiquidDisease Models, AnimalFatty LiverFood DeprivationLipoproteins, LDLLiverMaleMiceMice, Inbred C57BLPhosphatidylcholinesPhospholipidsTriglyceridesMammalia To reference this document use: http://resolver.tudelft.nl/uuid:04a14123-b5ef-4a3a-8cd3-9da8dfd65434 DOI https://doi.org/10.1016/j.bbalip.2007.07.007 TNO identifier 240207 ISSN 1388-1981 Source Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1771 (10), 1263-1270 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.