Title
Comparison of dose-responses of contact allergens using the guinea pig maximization test and the local lymph node assay
Author
van Och, F.M.M.
Vandebriel, R.J.
Prinsen, M.K.
de Jong, W.H.
Slob, W.
van Loveren, H.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
2001
Abstract
The guinea pig maximization test (GPMT) has been used as a method for the prediction of skin sensitizing potential for over 30 years. Besides hazard identification, risk assessment of sensitizing chemicals requires the assessment of potency. For the determination of potency based on lowest effective dose levels, dose-response studies are required. In the standard GPMT a single concentration is used for intracutaneous and topical induction and the assay provides a qualitative assessment of allergenicity. This paper presents data derived from quantitative evaluation of the sensitizing potency of chemicals in the GPMT, based on multiple concentrations. We performed the GPMT in accordance with the original procedure of Magnusson and Kligman; and included in this procedure a range of intradermal and topical concentrations for induction. Three allergens with different sensitizing potencies, diethylamine (DEA), tetramethyl thiuram disulfide (TMTD) and zinc dimethyl dithiocarbamate (ZDMC) were tested. The data obtained with this test procedure were compared to data we previously obtained using the local lymph node assay (LLNA). Both the GPMT and the LLNA showed dose response relationships for the three chemicals tested. For the chemicals tested, both tests differed in the relative potencies based on benchmark concentrations. While both tests ranked DEA as the least potent allergen, the GPMT ranked ZDMC more potent than TMTD, the reverse being found in the LLNA. The nature of the data provided in the LLNA makes it likely that benchmarks as defined with this test are more reliable than that defined in the GPMT. However, further validation with human data is necessary. © 2001 Elsevier Science Ireland Ltd. All rights reserved. Chemicals/CAS: Allergens; diethylamine, 109-89-7; Diethylamines; Thiram, 137-26-8; Ziram, 137-30-4
Subject
Nutrition
Allergens
Animals
Dermatitis, Allergic Contact
Diethylamines
Dose-Response Relationship, Immunologic
Edema
Erythema
Guinea Pigs
Local Lymph Node Assay
Male
Mice
Skin
Thiram
Time Factors
Ziram
To reference this document use:
http://resolver.tudelft.nl/uuid:0461441e-0156-43d6-8ede-885811d27e48
DOI
https://doi.org/10.1016/s0300-483x(01)00425-5
TNO identifier
87635
ISSN
0300-483X
Source
Toxicology, 167 (3), 207-215
Document type
article