Print Email Facebook Twitter Methods for the differential integrative omic analysis of plasma from a transgenic disease animal model Title Methods for the differential integrative omic analysis of plasma from a transgenic disease animal model Author Davidov, E. Clish, C.B. Oresic, M. Meys, M. Stochaj, W. Snell, P. Lavine, G. Londo, T.R. Adourian, A. Zhang, X. Johnston, M. Morel, N. Marple, E.W. Plasterer, T.N. Neumann, E. Verheij, E. Vogels, J.T.W.E. Havekes, L.M. van der Greef, J. Naylor, S. TNO Preventie en Gezondheid Publication year 2004 Abstract Multitiered quantitative analysis of biological systems is rapidly becoming the desired approach to study hierarchical functional interactions between proteins and metabolites. We describe here a novel systematic approach to analyze organisms with complex metabolic regulatory networks. By using precise analytical methods to measure biochemical constituents and their relative abundance in whole plasma of transgenic ApoE*3-Leiden mice and an isogenic wild-type control group, simultaneous snapshots of metabolic and protein states were obtained. Novel data processing and multivariate analysis tools such as Impurity Resolution Software (IMPRESS™) and Windows-based linear fit program (WINLIN™) were used to compare protein and metabolic profiles in parallel. Canonical correlations of the resulting data show quantitative relationships between heterogeneous components in the TG animals. These results, obtained solely from whole plasma analysis allowed us, in a rapid manner, to corroborate previous findings as well as find new events pertaining to dominant and peripheral events in lipoprotein metabolism of a genetically modified mammalian organism in relation to ApoE3, a key mediator of lipoprotein metabolism. Subject BiologyAnalytical researchBiomedical researchApolipoprotein E3LipidProteinAnalytic methodAnimal experimentAnimal modelComputer programControlled studyCorrelation analysisDNA modificationGene mutationInformation processingLipoprotein metabolismMetabolic regulationMetaboliteMouseMultivariate analysisNonhumanPlasmaQuantitative analysisTransgenic mouseAnimalsApolipoprotein E3Apolipoproteins EArteriosclerosisChromatography, LiquidCrosses, GeneticFemaleGenes, DominantGenetic TechniquesHumansHyperlipoproteinemiasLipid MetabolismLipoproteinsMagnetic Resonance SpectroscopyMaleMass SpectrometryMiceMice, Inbred C57BLMice, TransgenicMultivariate AnalysisMutationPeptidesPrincipal Component AnalysisProteinsSoftwareTime FactorsTrypsin To reference this document use: http://resolver.tudelft.nl/uuid:03a3d634-ca46-47ce-8391-81d5975cde09 TNO identifier 238201 ISSN 1536-2310 Source OMICS A Journal of Integrative Biology, 8 (4), 267-288 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.