Title
Methods for the differential integrative omic analysis of plasma from a transgenic disease animal model
Author
Davidov, E.
Clish, C.B.
Oresic, M.
Meys, M.
Stochaj, W.
Snell, P.
Lavine, G.
Londo, T.R.
Adourian, A.
Zhang, X.
Johnston, M.
Morel, N.
Marple, E.W.
Plasterer, T.N.
Neumann, E.
Verheij, E.
Vogels, J.T.W.E.
Havekes, L.M.
van der Greef, J.
Naylor, S.
TNO Preventie en Gezondheid
Publication year
2004
Abstract
Multitiered quantitative analysis of biological systems is rapidly becoming the desired approach to study hierarchical functional interactions between proteins and metabolites. We describe here a novel systematic approach to analyze organisms with complex metabolic regulatory networks. By using precise analytical methods to measure biochemical constituents and their relative abundance in whole plasma of transgenic ApoE*3-Leiden mice and an isogenic wild-type control group, simultaneous snapshots of metabolic and protein states were obtained. Novel data processing and multivariate analysis tools such as Impurity Resolution Software (IMPRESS™) and Windows-based linear fit program (WINLIN™) were used to compare protein and metabolic profiles in parallel. Canonical correlations of the resulting data show quantitative relationships between heterogeneous components in the TG animals. These results, obtained solely from whole plasma analysis allowed us, in a rapid manner, to corroborate previous findings as well as find new events pertaining to dominant and peripheral events in lipoprotein metabolism of a genetically modified mammalian organism in relation to ApoE3, a key mediator of lipoprotein metabolism.
Subject
Biology
Analytical research
Biomedical research
Apolipoprotein E3
Lipid
Protein
Analytic method
Animal experiment
Animal model
Computer program
Controlled study
Correlation analysis
DNA modification
Gene mutation
Information processing
Lipoprotein metabolism
Metabolic regulation
Metabolite
Mouse
Multivariate analysis
Nonhuman
Plasma
Quantitative analysis
Transgenic mouse
Animals
Apolipoprotein E3
Apolipoproteins E
Arteriosclerosis
Chromatography, Liquid
Crosses, Genetic
Female
Genes, Dominant
Genetic Techniques
Humans
Hyperlipoproteinemias
Lipid Metabolism
Lipoproteins
Magnetic Resonance Spectroscopy
Male
Mass Spectrometry
Mice
Mice, Inbred C57BL
Mice, Transgenic
Multivariate Analysis
Mutation
Peptides
Principal Component Analysis
Proteins
Software
Time Factors
Trypsin
To reference this document use:
http://resolver.tudelft.nl/uuid:03a3d634-ca46-47ce-8391-81d5975cde09
TNO identifier
238201
ISSN
1536-2310
Source
OMICS A Journal of Integrative Biology, 8 (4), 267-288
Document type
article