Print Email Facebook Twitter PET imaging of disease progression and treatment effects in the experimental autoimmune encephalomyelitis rat model Title PET imaging of disease progression and treatment effects in the experimental autoimmune encephalomyelitis rat model Author de Paula Faria, D. Vlaming, M.L.H. Copray, S.C.V.M. Tielen, F. Anthonijsz, H.J.A. Sijbesma, J.W.A. Buchpiguel, C.A. Dierckx, R.A.J.O. van der Hoorn, J.W.A. de Vries, E.F.J. Publication year 2014 Abstract The experimental autoimmune encephalomyelitis model is a model of multiple sclerosis that closely mimics the disease characteristics in humans. The main hallmarks of multiple sclerosis are neuroinflammation (microglia activation, monocyte invasion, and T-cell infiltration) and demyelination. PET imaging may be a useful noninvasive technique for monitoring disease progression and drug treatment efficacy in vivo. Methods: Experimental autoimmune encephalomyelitis was induced by myelin-oligodendrocyte glycoprotein immunization in female Dark Agouti rats. Experimental autoimmune encephalomyelitis rats were imaged at baseline and at days 6, 11, 15, and 19 after immunization to monitor monocyte and microglia activation (11C-PK11195) and demyelination ( 11C-MeDAS) during normal disease progression and during treatment with dexamethasone. Results: 11C-PK11195 PET detected activation of microglia and monocytes in the brain stem and spinal cord during disease progression. The uptake of 11C-PK11195 was elevated in dexamethasone-treated animals that had shown mild clinical symptoms that had resolved at the time of imaging. Demyelination was not detected by 11C-MeDAS PET, probably because of the small size of the lesions (average, 0.13 mm). Conclusion: PET imaging of neuroinflammation can be used to monitor disease progression and the consequences of treatment in the experimental autoimmune encephalomyelitis rat model. PET imaging was more sensitive than clinical symptoms for detecting inflammatory changes in the central nervous system. COPYRIGHT © 2014 by the Society of Nuclear Medicine and Molecular Imaging, Inc.Chemicals/CAS: dexamethasone acetate, 1177-87-3 Subject LifeMSB - Microbiology and Systems Biology MHR - Metabolic Health ResearchELSS - Earth, Life and Social SciencesBiomedical InnovationBiologyHealthy LivingDemyelinationMultiple sclerosisNeuroinflammationPET imagingDexamethasone acetateMeDAS c 11Myelin oligodendrocyte glycoproteinN sec butyl 1 (2 chlorophenyl) n methyl 3 isoquinolinecarboxamide c 11Radiopharmaceutical agentUnclassified drugAnimal cellAnimal experimentAnimal modelAnimal tissueBrain regionCell invasionControlled studyDemyelinationDisease courseDrug effectExperimental autoimmune encephalomyelitisFemaleIn vivo studyMicrogliaMonocyteNeuroimagingNonhumanPET scannerPositron emission tomographyPriority journalRatScoring system To reference this document use: http://resolver.tudelft.nl/uuid:01f6aa40-bc61-4696-9d49-58d01c02f699 DOI https://doi.org/10.2967/jnumed.114.137216 TNO identifier 513449 ISSN 0161-5505 Source Journal of Nuclear Medicine, 55 (8), 1330-1335 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.