Print Email Facebook Twitter Increased hepatic insulin sensitivity together with decreased hepatic triglyceride stores in hormone-sensitive lipase-deficient mice Title Increased hepatic insulin sensitivity together with decreased hepatic triglyceride stores in hormone-sensitive lipase-deficient mice Author Voshol, P.J. Haemmerle, G. Ouwens, D.M. Zimmermann, R. Zechner, R. Teusink, B. Maassen, J.A. Havekes, L.M. Romijn, J.A. Publication year 2003 Abstract Hormone-sensitive lipase (HSL) is a major enzyme for triglyceride (TG) lipolysis in adipose tissue. In HSL-knockout mice, plasma free fatty acid and TG levels are low, associated with low liver TG content. Because a decreased hepatic insulin sensitivity has been reported to be associated with high liver TG levels, our aim was to determine whether a hepatic TG content lower than normal, as observed in HSL-knockout mice, leads to increased hepatic insulin sensitivity. Therefore, hyperinsulinemic clamp experiments in combination with D-3H. glucose were used. Furthermore, hepatic insulin receptor and phosphorylated protein kinase B (PKB-P)/akt were analyzed by Western blotting. No significant differences where observed in insulin-mediated whole-body glucose uptake between HSL-knockout and control mice. Interestingly, hepatic insulin sensitivity of HSL-knockout mice was increased, because insulin caused a greater reduction in endogenous glucose production (∼71% compared with ∼31% in control mice; P < 0.05), despite decreased plasma adiponectin levels. PKB/akt phosphorylation and phosphatidylinositol-3-kinase activity was significantly higher in livers of HSL-knockout mice after insulin stimulation. In HSL-knockout mice, reduced hepatic TG stores result in an increased suppressive effect of insulin on hepatic glucose production, in line with an increased hepatic PKB-P/akt and phosphatidylinositol-3 kinase activity. Thus, hepatic insulin sensitivity is indeed increased after reducing hepatic TG stores below normal. Subject HealthGlucoseHormone sensitive lipaseInsulin receptorProtein kinase BTriacylglycerolTriacylglycerol lipaseUnclassified drugAnimal tissueControlled studyEnzyme activityEnzyme deficiencyEnzyme mechanismEnzyme phosphorylationFatty acid blood levelGluconeogenesisGlucose transportHormone sensitivityHyperinsulinemiaInsulin sensitivityKnockout mouseLipid liver levelLipolysisMouseNonhumanWestern blottingAdiponectinAnimalsBlood GlucoseCholesterolCholesterol EsteraseFastingFatty Acids, NonesterifiedGlucoseInsulinIntercellular Signaling Peptides and ProteinsLiverMaleMiceMice, KnockoutMuscle, SkeletalProteinsReceptor, InsulinTriglycerides To reference this document use: http://resolver.tudelft.nl/uuid:01ad5532-d637-44f7-ac5d-b99faa97cda4 DOI https://doi.org/10.1210/en.2002-0036 TNO identifier 237212 ISSN 0013-7227 Source Endocrinology, 144 (8), 3456-3462 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.