Print Email Facebook Twitter Instrument and process independent binning and baseline correction methods for liquid chromatography-high resolution-mass spectrometry deconvolution Title Instrument and process independent binning and baseline correction methods for liquid chromatography-high resolution-mass spectrometry deconvolution Author Krishnan, S. Vogels, J.T.W.E. Coulier, L. Bas, R.C. Hendriks, M.W.B. Hankemeier, T. Thissen, U. Publication year 2012 Abstract Setting appropriate bin sizes to aggregate hyphenated high-resolution mass spectrometry data, belonging to similar mass over charge (m/. z) channels, is vital to metabolite quantification and further identification. In a high-resolution mass spectrometer when mass accuracy (ppm) varies as a function of molecular mass, which usually is the case while reading m/. z from low to high values, it becomes a challenge to determine suitable bin sizes satisfying all m/. z ranges. Similarly, the chromatographic process within a hyphenated system, like any other controlled processes, introduces some process driven systematic behavior that ultimately distorts the mass chromatogram signal. This is especially seen in liquid chromatogram-mass spectrometry (LC-MS) measurements where the gradient of the solvent and the washing step cycle-part of the chromatographic process, produce a mass chromatogram with a non-uniform baseline along the retention time axis. Hence prior to any automatic signal decomposition techniques like deconvolution, it is a equally vital to perform the baseline correction step for absolute metabolite quantification. This paper will discuss an instrument and process independent solution to the binning and the baseline correction problem discussed above, seen together, as an effective pre-processing step toward liquid chromatography-high resolution-mass spectrometry (LC-HR-MS) data deconvolution. © 2012 Elsevier B.V.. Subject Life Life Triskelion BVMSB - Microbiology and Systems Biology QS - Quality & Safety ARPC - Analytical Research (Pharm & Chemistry)EELS - Earth, Environmental and Life Sciences TNO BedrijvenHealthy for LifeChemistryHealthy LivingAccurate mass binningBaseline correctionDeconvolutionLiquid chromatography-mass spectrometryPre-processing To reference this document use: http://resolver.tudelft.nl/uuid:016f85e7-4ac5-4bc3-9706-50e9d2e0321f DOI https://doi.org/10.1016/j.aca.2012.06.01 TNO identifier 462854 ISSN 0003-2670 Source Analytica Chimica Acta, 740, 12-19 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.