Print Email Facebook Twitter Toll-like receptor 2 activation by β2→1-fructans protects barrier function of t84 human intestinal epithelial cells in a chain length-dependent manner Title Toll-like receptor 2 activation by β2→1-fructans protects barrier function of t84 human intestinal epithelial cells in a chain length-dependent manner Author Vogt, L.M. Meyer, D. Pullens, G. Faas, M.M. Venema, K. Ramasamy, U. Schols, H.A. de Vos, P. Publication year 2014 Abstract Dietary fiber intake is associated with lower incidence and mortality from disease, but the underlying mechanisms of these protective effects are unclear.We hypothesized that β2→1-fructan dietary fibers confer protection on intestinal epithelial cell barrier function via Toll-like receptor 2 (TLR2), and we studied whether β2→1-fructan chain-length differences affect this process. T84 human intestinal epithelial cell monolayers were incubated with 4 β2→1-fructan formulations of different chain-length compositions and were stimulated with the proinflammatory phorbol 12-myristate 13-acetate (PMA). Transepithelial electrical resistance (TEER) was analyzed by electric cell substrate impedance sensing (ECIS) as a measure for tight junction-mediated barrier function. To confirm TLR2 involvement in barrier modulation by β2→1-fructans, ECIS experiments were repeated using TLR2 blocking antibody. After preincubation of T84 cells with short-chain β2→1-fructans, the decrease in TEER as induced by PMA (62.3 ± 5.2%, P < 0.001) was strongly attenuated (15.2 ± 8.8%, P < 0.01). However, when PMA was applied first, no effect on recovery was observed during addition of the fructans. By blocking TLR2 on the T84 cells, the protective effect of short-chain β2→1-fructans was substantially inhibited. Stimulation of human embryonic kidney human TLR2 reporter cells with β2→1-fructans induced activation of nuclear factor kappa-light-chain-enhancer of activated B cells, confirming that β2→1-fructans are specific ligands for TLR2. To conclude, β2→1-fructans exert time-dependent and chain length-dependent protective effects on the T84 intestinal epithelial cell barrier mediated via TLR2. These results suggest that TLR2 located on intestinal epithelial cells could be a target of β2→1-fructan-mediated health effects. © 2014 American Society for Nutrition.Chemicals/CAS: inulinase, 9025-67-6; phorbol 13 acetate 12 myristate, 16561-29-8; toll like receptor 2, 203811-81-8 Subject LifeMSB - Microbiology and Systems BiologyELSS - Earth, Life and Social SciencesBiomedical InnovationBiologyHealthy LivingBocking antibodyInulinasePhorbol 13 acetate 12 myristateReceptor activator of nuclear factor kappa BShort chain fatty acidToll like receptor 2B lymphocyteCell functionCell interactionCell protectionColon carcinomaDietary fiberElectric cell substrate impedance sensingElectric resistanceEnzyme activationEnzyme substrateHumanHuman cellImpedanceIntestine epithelium cellIntestine functionPermeability barrierRegulatory mechanismTight junctionTransepithelial electrical resistance To reference this document use: http://resolver.tudelft.nl/uuid:015fd3e0-6545-4f0c-98f9-eeab45e9b442 DOI https://doi.org/10.3945/jn.114.191643 TNO identifier 513427 ISSN 1541-6100 Source Journal of Nutrition, 144 (7), 1002-1008 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.