Title
Hyperoxia and local organ blood flow in the developing chick embryo
Author
van Golde, J.M.C.G.
Mulder, T.A.L.M.
Scheve, E.
Prinzen, F.W.
Blanco, C.E.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1999
Abstract
1. Hyperoxia can cause local vasoconstriction in adult animal organs as a protective mechanism against hyperoxia-induced toxicity. It is not known at what time during development this vasoconstrictor capacity is present. Therefore, we measured the cardiac output (GO) distribution in different organs during a period of acute hyperoxia (100% O2) in the developing chick embryo. 2. Fertile eggs mere divided into five incubation time groups (10 and 11, 12 and 13, 14 and 15, 16 and 17, and 18 and 19 days of a normal incubation time of 21 days). Eggs mere opened at the air cell and a catheter was inserted into a branch of the chorioallantoic vein for injections of 15 μm fluorescent microspheres during normoxia and at the end of 5 min (test group 1; n = 39) or 20 min (test group 2; n = 21) of hyperoxia exposure (100% O2). The fraction of CO to an organ was calculated as the fluorescence of the organ sample divided by the sum of the fluorescence of all organs. 3. Only in 18- and 19-day-old embryos did hyperoxia cause a decrease in the fractions of CO to the heart and carcass, and an increase in those to the yolk-sac and chorioallantoic membrane. This response was more pronounced after 20 min (test group 2) than after 5 min (test group 1) of hyperoxia with an additional decrease in the fractions of CO to the brain, intestine and liver (test group 2). 4. These data indicate that local mechanisms for hyperoxia-induced vasoconstriction in the heart, brain, liver, intestine and carcass develop late, during the final 15% of the incubation period, in the developing chick embryo.
Subject
Nutrition
Microsphere
Animal tissue
Chick embryo
Chorioallantois
Egg
embryo
Embryo development
Fluorescence
Heart output
Hyperoxia
Incubation time
Nonhuman
Organ blood flow
Priority journal
Yolk sac
Animals
Cardiac Output
Chick Embryo
Fluorescent Dyes
Hyperoxia
Microspheres
Oxygen
Oxygen Consumption
Regional Blood Flow
Vasoconstriction
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TNO identifier
234948
ISSN
0022-3751
Source
Journal of Physiology, 515 (1), 243-248
Document type
article