Title
Isolation and characterization of promoter regions from Streptococcus gordonii CH1
Author
Vriesema, A.J.M.
Dankert, J.
Zaat, S.A.J.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1999
Abstract
We aimed to identify transcription signal sequences from Streptococcus gordonii strain CH1 by random chromosomal cloning. Five genomic fragments from a Sau3A digest, which constitutively activated transcription of a promoterless spectinomycin resistance gene in this strain, were isolated and characterized. Additionally, one promoter fragment was isolated that was specifically activated under iron-limiting conditions. A sequence motif with similarity to the consensus for Fur-binding regulatory DNA sequences (Fur box) in Escherichia coli was detected within the putative promoter region. The open reading frame downstream of this region possibly encodes a transmembrane protein involved in iron uptake.
Subject
Nutrition
Bacterial DNA
Iron
Membrane protein
Bacterial gene
Bacterial virulence
Bacterium isolation
DNA sequence
Genetic transcription
Molecular cloning
Nonhuman
Nucleotide sequence
Open reading frame
Opportunistic infection
Priority journal
Promoter region
Streptococcus gordonii
Amino Acid Sequence
Base Sequence
DNA, Bacterial
Gene Expression Regulation, Bacterial
Iron
Molecular Sequence Data
Promoter Regions (Genetics)
Sequence Analysis, DNA
Streptococcus
Transcription, Genetic
Bacteria (microorganisms)
Escherichia coli
Posibacteria
Prokaryota
Streptococcus gordonii
To reference this document use:
http://resolver.tudelft.nl/uuid:00b05076-3aa1-4721-999d-25f530d11ae4
DOI
https://doi.org/10.1007/s002849900466
TNO identifier
235218
ISSN
0343-8651
Source
Current Microbiology, 39 (6), 321-326
Document type
article