Title
Tailoring fiber diameter in electrospun poly(ε-Caprolactone) scaffolds for optimal cellular infiltration in cardiovascular tissue engineering
Author
Balguid, A.
Mol, A.
van Marion, M.H.
Bank, R.A.
Bouten, C.V.C.
Baaijens, F.P.T.
TNO Kwaliteit van Leven
Publication year
2009
Abstract
Despite the attractive features of nanofibrous scaffolds for cell attachment in tissue-engineering (TE) applications, impeded cell ingrowth has been reported in electrospun scaffolds. Previous findings have shown that the scaffold can function as a sieve, keeping cells on the scaffold surface, and that cell migration into the scaffold does not occur in time. Because fiber diameter is directly related to the pore size of an electrospun scaffold, the objective of this study was to systematically evaluate how cell delivery can be optimized by tailoring the fiber diameter of electrospun poly(ε- caprolactone) (PCL) scaffolds. Five groups of electrospun PCL scaffolds with increasing average fiber diameters (3.4-12.1μm) were seeded with human venous myofibroblasts. Cell distribution was analyzed after 3 days of culture. Cell penetration increased proportionally with increasing fiber diameter. Unobstructed delivery of cells was observed exclusively in the scaffold with the largest fiber diameter (12.1 μm). This scaffold was subsequently evaluated in a 4-week TE experiment and compared with a poly(glycolic acid)-poly(4- hydroxybutyrate) scaffold, a standard scaffold used successfully in cardiovascular tissue engineering applications. The PCL constructs showed homogeneous tissue formation and sufficient matrix deposition. In conclusion, fiber diameter is a crucial parameter to allow for homogeneous cell delivery in electrospun scaffolds. The optimal electrospun scaffold geometry, however, is not generic and should be adjusted to cell size. © 2009, Mary Ann Liebert, Inc.
Subject
Biology
Biomedical Research
4-hydroxybutyrate
Average fiber diameters
Caprolactone
Cardiovascular tissue engineerings
Cell attachments
Cell deliveries
Cell distributions
Cell ingrowths
Cell migrations
Cell penetrations
Cell sizes
Cellular infiltrations
Crucial parameters
Electrospun
Fiber diameters
Homogeneous tissues
Myofibroblasts
Nanofibrous scaffolds
Poly(glycolic acid)
Sufficient matrixes
Applications
Cell culture
Cells
Fibers
Tissue engineering
Scaffolds
polycaprolactone
polyglycolic acid
tissue scaffold
article
cell infiltration
cell membrane permeability
cell transport
cellular distribution
controlled study
fiber
human
human cell
human cell culture
myofibroblast
priority journal
tissue engineering
vein
To reference this document use:
http://resolver.tudelft.nl/uuid:ee3ca7b1-7959-4ba3-b275-4c5245ff2a6c
DOI
https://doi.org/10.1089/ten.tea.2007.0294
TNO identifier
241400
ISSN
1937-3341
Source
Tissue Engineering - Part A, 15 (2), 437-444
Document type
article