Title
Purification of a novel fructosyltransferase from Lactobacillus reuteri strain 121 and characterization of the levan produced
Author
Hijum, S.A.F.T.
Bonting, K.
van der Maarel, M.J.E.V.
Dijkhuizen, L.
TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO
Publication year
2001
Abstract
Fructosyltransferase (FTF) enzymes have been characterized from various Gram-positive bacteria, but not from Lactobacillus sp. In a screening of 182 lactobacilli for polysaccharide production only one strain, Lactobacillus reuteri strain 121, was found to produce a fructan being a levan. Here we report the first-time identification and biochemical characterization of a Lactobacillus FTF enzyme. When incubated with sucrose the enzyme produced a levan that is identical to that produced by Lb. reuteri strain 121 cells. © 2001 Pubblished by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies. Chemicals/CAS: Fructans; Hexosyltransferases, EC 2.4.1.-; levan fructotransferase, EC 2.4.1.-; levan, 9013-95-0; levansucrase, EC 2.4.1.10; Polysaccharides, Bacterial; Sucrose, 57-50-1
Subject
Biology
Amino Acid Sequence
Fructans
Hexosyltransferases
Lactobacillus
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Molecular Weight
Polysaccharides, Bacterial
Sequence Alignment
Sucrose
Lactobacillus
Lactobacillus reuteri
Lactobacillus sp.
Posibacteria
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TNO identifier
72488
Source
FEMS Microbiology Letters, 205, 323-328
Document type
article