Title
A tormentor in the quest for plant p53-like proteins
Author
Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding
Korthout, H.A.A.J
Caspers, M.P.M
Kottenhagen, M.J.
Helmer, Q.
Wang, M.
Publication year
2002
Abstract
Over the past few years the presence of p53-like proteins in plants was frequently reported, by using the monoclonal antibody Pab240. By means of protein purification and screening a cDNA library, a Pab240 cross-reacting protein and a cDNA clone were isolated from barley. Peptide- and DNA-sequence analysis identified one and the same protein: 2-oxoglutarate dehydrogenase. Sequence analysis of 2-oxoglutarate dehydrogenase revealed that the protein contains a perfect Pab240 epitope. In barley, the 110 kDa oxoglutarate dehydrogenase was degraded during isolation to a 53 kDa Pab240 cross-reacting polypeptide, thereby mimicking curiously p53-like properties. © 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved. Chemicals/CAS: Ketoglutarate Dehydrogenase Complex, EC 1.2.4.2; Plant Proteins; Tumor Suppressor Protein p53
Subject
2-Oxoglutarate dehydrogenase
Barley
Immuno recognition
Pab240
cross reacting antibody
Epitope
Monoclonal antibody
Monoclonal antibody pab240
Oxoglutarate dehydrogenase
Protein p53
Unclassified drug
Vegetable protein
Amino acid sequence
Controlled study
DNA library
DNA screening
DNA sequence
Molecular cloning
Molecular weight
Nonhuman
Protein analysis
Protein degradation
Protein isolation
Protein purification
Sequence analysis
Amino Acid Sequence
Base Sequence
Hordeum
Ketoglutarate Dehydrogenase Complex
Molecular Sequence Data
Plant Proteins
Sequence Alignment
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Tumor Suppressor Protein p53
Hordeum vulgare subsp. vulgare
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DOI
https://doi.org/10.1016/s0014-5793(02)03113-7
TNO identifier
236660
ISSN
0014-5793
Source
FEBS Letters, 526 (526), 53-57
Document type
article