Title
Cytogenetic findings in mouse multiple myeloma and Waldenström's macroglobulinemia
Author
van den Akker, Th.W.
Radl, J.
Franken-Postma, E.
Hagemeijer, A.
Gaubius Instituut TNO
Publication year
1996
Abstract
Multiple myeloma (MM) and Waldenström's macroglobulinemia-like lymphoma (MW) appear spontaneously in C57BL/KaLwRij mice at a frequency of 0.5% and 0.2%, respectively. They can readily be propagated by intravenous transfer of mainly bone marrow or spleen cells into syngeneic recipients. Previous studies demonstrated that these mouse malignant monoclonal gammopathies (MMG) show clinical and biologic features that closely resemble those of the corresponding human diseases and thus could be used as experimental models. We report on cytogenetic analysis of two mouse MW and five MM in vivo cell lines of the 5TMM series propagated in syngeneic mice. These studies demonstrated clonal abnormalities in all cell lines, hyperdiploid karyotype in both MW and one MM lines, and hypotriploidy, hypertriploidy, or hypotetraploidy in the other lines. Structural abnormalities of chromosome 15 were observed in all MM lines. In the five MM lines, frequent rearrangements were also found for chromosome numbers 1, 2, 5, and 12. A single chromosomal abnormality, as found in induced mouse plasmacytomas and resembling Burkitt lymphoma, was not found in mouse MM and MW. It was concluded that spontaneously originating C57BL MM of the 5T series is a better model for human MM than pristane-induced BALB/c or NZB plasmacytoma.
Subject
Biology
animal cell
animal experiment
animal model
animal tissue
article
bone marrow cell
burkitt lymphoma
cell transfer
chromosome aberration
chromosome analysis
controlled study
gammopathy
gene rearrangement
mouse
multiple myeloma
nonhuman
plasmacytoma
priority journal
triploidy
waldenstroem macroglobulinemia
Animals
Chromosome Aberrations
Female
Genes, Immunoglobulin
Humans
Karyotyping
Male
Mice
Mice, Inbred C57BL
Multiple Myeloma
Neoplasm Transplantation
Tumor Cells, Cultured
Waldenstrom Macroglobulinemia
To reference this document use:
http://resolver.tudelft.nl/uuid:e06281b6-a3b0-4667-9b10-c2374c5eda2a
DOI
https://doi.org/10.1016/0165-4608(95)00169-7
TNO identifier
233203
ISSN
0165-4608
Source
Cancer Genetics and Cytogenetics, 86 (2), 156-161
Document type
article