Title
Expression of accessory molecules and cytokines in acute EAE in marmoset monkeys (Callithrix jacchus)
Author
Laman, J.D.
van Meurs, M.
Schellekens, M.M.
de Boer, M.
Melchers, B.
Massacesi, L.
Lassmann, H.
Claassen, E.
't Hart, B.A.
TNO Preventie en Gezondheid
Publication year
1998
Abstract
Accessory molecules and cytokines are involved in the immunopathogenesis of multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE) in rodent models, and are potential targets for immunotherapy. Evaluation of such experimental therapies requires appropriate animal models. Therefore, we analysed the expression of selected accessory molecules and cytokines in the brain of marmoset monkeys (Callithrix jacchus) with acute EAE, a newly described non-human primate model for MS. All animals experienced active disease clinically and histopathologically with strong resemblance to MS. Perivascular infiltrates of mononuclear cells showed abundant expression of CD40. CD40 was expressed on macrophages, indicating that T cell priming and macrophage effector functions may result from local CD40-CD40L interactions. CD40 ligand (CD40L) and B7-2 (CD86) were also expressed, but to a lower extent, while B7-1 (CD80) expression was limited. Both pro-inflammatory and anti-inflammatory cytokines were produced within individual lesions during active disease (IFN-α, IFN-γ, TNF-α, IL-1 α, IL-1β, IL-2, IL-4, IL-10 and IL-12). This suggests that relative levels rather than sequential expression of Th1- and Th2-type cytokines determine disease activity. These findings demonstrate the value of EAE in marmoset monkeys as a model to assess the role of accessory molecules and cytokines in multiple sclerosis, and to evaluate targeted intervention.
Subject
Health
CD40
CD80
CD86
Macrophages
Multiple sclerosis
Non-human primates
Acid Phosphatase
Animals
Antigens, CD
Antigens, CD40
Antigens, CD80
Antigens, CD86
Brain
Brain Chemistry
Callithrix
Cytokines
Disease Models, Animal
Encephalomyelitis, Autoimmune, Experimental
Female
Histocompatibility Antigens Class II
HLA-DR Antigens
Interferon Type II
Interferon-alpha
Interleukin-1
Interleukin-10
Interleukin-12
Interleukin-2
Interleukin-4
Macrophages
Male
Membrane Glycoproteins
Multiple Sclerosis
Tumor Necrosis Factor-alpha
To reference this document use:
http://resolver.tudelft.nl/uuid:dd331cc2-1269-46bd-a460-c7ee1bba0649
DOI
https://doi.org/10.1016/s0165-5728(98)00024-1
TNO identifier
234484
ISSN
0165-5728
Source
Journal of Neuroimmunology, 86 (1), 30-45
Document type
article