Title
Development of a detection system for histidine decarboxylating lactic acid bacteria based on DNA probes, PCR and activity test
Author
Le Jeune, C.
Lonvaud-Funel, A.
ten Brink, B.
Hofstra, H.
van der Vossen, J.M.B.M.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1995
Abstract
On the basis of the comparison of the nucleotide sequences of the histidine decarboxylase genes (hdcA) of Lactobacillus 30A and Clostridium perfringens and the amino acid sequences of these histidine decarboxylases and those of Lactobacillus buchneri and Micrococcus, oligonucleotides unique to the hdcA genes were synthesized and used in PCR. All histidine-decarboxylating lactic acid bacteria gave a signal with primer set JV16HC/JV17HC in PCR. In addition to this primer set, CL1/CL2 and CL1/JV17HC were also useful for the detection of histamine-forming Leuconostoc oenos strains in PCR. The 150 base pair amplification product of the decarboxylating Leuc. oenos strain generated with primer set CL1/CL2 was sequenced. Alignment studies showed a high degree of relatedness among the hdcA gene products of Gram-positive bacteria. The amplification products of the hdcA genes from Lact. buchneri and Leuc. oenos were used to serve as a DNA probe in hybridization studies. All histidine-decarboxylating lactic acid bacteria gave a hybridization signal with the DNA probes. In hybridization only one false-positive signal with a Lactobacillus lindneri strain was observed, which was anticipated to contain a truncated hdcA gene. In addition to these DNA probe tests, a simple and reliable activity test is presented, which can be used during starter selection to test strains for histidine decarboxylase activity.
Subject
Nutrition
Amino Acid Sequence
Animal
Base Sequence
Clostridium perfringens
Comparative Study
DNA Probes
Evaluation Studies
Food Microbiology
Histamine
Histidine
Histidine Decarboxylase
Lactates
Lactic Acid
Lactobacillus
Leuconostoc
Molecular Sequence Data
Polymerase Chain Reaction
Sequence Homology, Nucleic Acid
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http://resolver.tudelft.nl/uuid:d85f8b6d-74a2-405d-912d-1c38867dbc1a
TNO identifier
67249
ISSN
0021-8847
Source
Journal of Applied Bacteriology, 78 (3), 316-326
Document type
article