Title
Primary structure of a lipoxygenase from barley grain as deduced from its cDNA sequence
Author
van Mechelen, J.R.
Smits, M.
Douma, A.C.
Rouster, J.
Cameron-Mills, V.
Heidekamp, F.
Valk, B.E.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1995
Abstract
A full length cDNA sequence for a barley grain lipoxygenase was obtained. It includes a 5' untranslated region of 69 nucleotides, an open reading frame of 2586 nucleotides encoding a protein of 862 amino acid residues and a 3' untranslated region of 142 nucleotides. The molecular mass of the encoded polypeptide was calculated to be 96.392. Its amino acid sequence shows a high homology with that of other plant lipoxygenases identified to date.
Subject
Nutrition
Barley
CDNA cloning
Hordeum vulgare L.
Inverse PCR cloning
LOX
Plant lipoxygenase
Lipoxygenase
Barley
Dna sequence
Enzyme analysis
Phytochemistry
Priority journal
Protein analysis
Amino Acid Sequence
Base Sequence
DNA, Complementary
Hordeum
Lipoxygenase
Molecular Sequence Data
Polymerase Chain Reaction
Sequence Analysis
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DOI
https://doi.org/10.1016/0005-2760(94)00231-m
TNO identifier
232921
ISSN
0005-2760
Source
Biochimica et Biophysica Acta - Lipids and Lipid Metabolism, 1254 (2), 221-225
Document type
article