Print Email Facebook Twitter Analysis of temporal gene expression during Bacillus subtilis spore germination and outgrowth Title Analysis of temporal gene expression during Bacillus subtilis spore germination and outgrowth Author Keijser, B.J.F. ter Beek, A. Rauwerda, H. Schuren, F. Montijn, R. van der Spek, H. Brul, S. TNO Kwaliteit van Leven Publication year 2007 Abstract Bacillus subtilis forms dormant spores upon nutrient depletion. Under favorable environmental conditions, the spore breaks its dormancy and resumes growth in a process called spore germination and outgrowth. To elucidate the physiological processes that occur during the transition of the dormant spore to an actively growing vegetative cell, we studied this process in a time-dependent manner by a combination of microscopy, analysis of extracellular metabolites, and a genome-wide analysis of transcription. The results indicate the presence of abundant levels of late sporulation transcripts in dormant spores. In addition, the results suggest the existence of a complex and well-regulated spore outgrowth program, involving the temporal expression of at least 30% of the B. subtilis genome. Copyright © 2007, American Society for Microbiology. All Rights Reserved. Subject BiologyBiotechnologyCell divisionCell growthDNA repairDNA replicationMicroscopySpore germinationSporogenesisTranscription regulationBacillus subtilisGene ExpressionGene Expression ProfilingOligonucleotide Array Sequence AnalysisRNA, BacterialRNA, MessengerSpores, BacterialTime FactorsTranscription, GeneticBacillus subtilis To reference this document use: http://resolver.tudelft.nl/uuid:d2849b7d-da38-4f13-9136-3a993bf91cea DOI https://doi.org/10.1128/jb.01736-06 TNO identifier 239955 ISSN 0021-9193 Source Journal of Bacteriology, 189 (9), 3624-3634 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.