Title
Retrospective detection of exposure to sulfur mustard: Improvements on an assay for liquid chromatography-tandem mass spectrometry analysis of albumin/sulfur mustard adducts
Author
Prins Maurits Laboratorium TNO
Noort, D.
Fidder, A.
Hulst, A.G.
Woolfitt, A.R.
Ash, D.
Barr, J.R.
Publication year
2004
Abstract
We here report on the further development of the method comprising the pronase digestion of albumin alkylated by sulfur mustard and the subsequent mass spectrometric analysis of an adducted tripeptide. This includes significant improvements in both the albumin isolation procedure and the automation of the microliquid chromatography-electrospray-tandem mass spectrometric analysis. We also report on the results of a small reference range study, in which we have established that there are no detectable interferences in sera from unexposed individuals.
Subject
Tripeptide
Alkylation
Autoanalysis
Blood analysis
Chemical analysis
Chemical warfare
Controlled study
Digestion
Human
Human tissue
Liquid chromatography
Mass spectrometry
Protein isolation
Serum
Standard
Albumins
Alkylation
Chemical warfare agents
Chromatography, Affinity
Environmental exposure
Humans
Reference values
Retrospective studies
Mass spectrometry
Electrospray ionization
Mustard gas, 505-60-2
Pronase, 9036-06-0
Albumins
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http://resolver.tudelft.nl/uuid:cd4fb3f1-3ce6-4312-9db4-3d8735c55807
TNO identifier
237891
ISSN
0146-4760
Source
Journal of Analytical Toxicology, 28 (28), 333-338
Document type
article