Title
Substitution patterns in methylated potato starch as revealed from the structure and composition of fragments in enzymatic digests
Author
Steeneken, P.A.M.
Tas, A.C.
Woortman, A.J.J.
Sanders, P.
Mijland, P.J.H.C.
de Weijs, L.G.R.
TNO Kwaliteit van Leven
Publication year
2008
Abstract
The effect of the granule structure on the methylation of starch was investigated by comparing the substitution patterns of potato starch methylated in granular suspension and in solution to DS 0.3. Substitution patterns were analyzed by successive digestion with α-amylase and amyloglucosidase, fractionation of the resulting malto-oligosaccharide mixture by GPC on a preparative scale, and characterization of the fractions by GLC and MALDI-MS. The mass composition of fractions with intermediate and higher degree of polymerization was indicative of enhanced clustering of substituents in granular methyl starch. On the contrary, the composition of the smaller saccharides was governed by enzyme specificity, which was also reflected in strong deviations in their monomer composition. A sequencing study on selected 'pure' small saccharides confirmed and complemented previous conclusions on enzyme specificity. © 2008 Elsevier Ltd. All rights reserved.
Subject
Nutrition
Analytical research
Enzymatic digestibility
MALDI
Methylation
Starch
Substitution pattern
Chemical reactions
Enzymes
Food additives
Monomers
Polymers
Structure (composition)
Sugars
Amyloglucosidase
Degree of polymerization
Enzymatic digestibility
Enzyme specificity
Granule structure
MALDI
Mass compositions
Methylation
Monomer compositions
Potato starch
Substitution pattern
Substitution patterns
Starch
amylase
carbohydrate
glucan 1,4 alpha glucosidase
oligosaccharide
potato starch
article
carbohydrate analysis
enzyme chemistry
enzyme specificity
fractionation
gas liquid chromatography
matrix assisted laser desorption ionization time of flight mass spectrometry
methylation
polymerization
priority journal
alpha-Amylases
Chromatography, Liquid
Glucan 1,4-alpha-Glucosidase
Methylation
Oligosaccharides
Solanum tuberosum
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Starch
Substrate Specificity
Solanum tuberosum
To reference this document use:
http://resolver.tudelft.nl/uuid:c774b30a-6cda-498e-aa5a-6ebd6251df17
DOI
https://doi.org/10.1016/j.carres.2008.06.029
TNO identifier
241021
ISSN
0008-6215
Source
Carbohydrate Research, 343 (14), 2411-2416
Document type
article