Title
Late rather than early responses of human dendritic cells highlight selective induction of cytokines, chemokines and growth factors by probiotic bacteria.
Author
Verbeek, R.
Bsibsi, M.
Plomp, A.
van Neerven, R.J.
te Biesebeke, R.
van Noort, J.M.
TNO Kwaliteit van Leven
Publication year
2010
Abstract
The probiotic properties of commensal bacteria including lactobacilli and bifidobacteria are likely to be determined at least in part by their effects on dendritic cells. Like traditional immune stimulants such as lipopolysaccharides (LPS), probiotic bacteria promote maturation of cultured human dendritic cells (DC) by inducing elevated expression of MHC-II and co-stimulatory molecules. Different effects have been reported on cytokine induction, especially of major regulatory cytokines such as TNF-a, IL-12 and IL-10. Yet, these previous analyses have failed to reveal consistent differences between such effects of probiotics on the one hand, and of LPS on the other. Selective response markers for probiotics, however, would be important for our understanding of their biological properties and for a rational selection of strains for in vivo studies. In this study, we compared in detail both early and late effects on cultured human DC of 4 different probiotics with those of LPS. At the early stages of stimulation, all stimuli induced qualitatively very similar responses in DC at the level of surface markers and secretion of cytokines and chemokines. A lower immune stimulatory effect was observed by Bifidobacterium animalis BB-12 as compared to lactobacilli. Late responses, on the other hand, tended to diverge. Microarray transcript profiling for 268 cytokines, chemokines, growth factors and their receptors after 2 days of culture revealed various transcripts to be selectively induced by certain probiotics but not LPS. Our data indicate that late rather than early DC responses may be helpful to clarify the divergent biological effects of probiotics on human innate immune responses.
Subject
Life
MHR - Metabolic Health Research
EELS - Earth, Environmental and Life Sciences
Biomedical Innovation
Pharmacology Health
Healthy Living
cytokine
probiotic agent
article
Bifidobacterium
cell culture
dendritic cell
Escherichia coli
human
immunology
Lactobacillus
secretion
Bifidobacterium
Cells, Cultured
Cytokines
Dendritic Cells
Escherichia coli
Humans
Lactobacillus
Probiotics
Chemicals/CAS: Cytokines
To reference this document use:
http://resolver.tudelft.nl/uuid:c6f437a9-9c34-4cec-aee0-e14af3a6afb1
TNO identifier
446672
ISSN
1876-2891
Source
Beneficial microbes, 1 (2), 109-119
Document type
article