Title
Toxicogenomics concepts and applications to study hepatic effects of food additives and chemicals
Author
Stierum, R.
Heijne, W.
Kienhuis, A.
van Ommen, B.
Groten, J.
TNO Kwaliteit van Leven
Publication year
2005
Abstract
Transcriptomics, proteomics and metabolomics are genomics technologies with great potential in toxicological sciences. Toxicogenomics involves the integration of conventional toxicological examinations with gene, protein or metabolite expression profiles. An overview together with selected examples of the possibilities of genomics in toxicology is given. The expectations raised by toxicogenomics are earlier and more sensitive detection of toxicity. Furthermore, toxicogenomics will provide a better understanding of the mechanism of toxicity and may facilitate the prediction of toxicity of unknown compounds. Mechanism-based markers of toxicity can be discovered and improved interspecies and in vitro-in vivo extrapolations will drive model developments in toxicology. Toxicological assessment of chemical mixtures will benefit from the new molecular biological tools. In our laboratory, toxicogenomics is predominantly applied for elucidation of mechanisms of action and discovery of novel pathway-supported mechanism-based markers of liver toxicity. In addition, we aim to integrate transcriptome, proteome and metabolome data, supported by bioinformatics to develop a systems biology approach for toxicology. Transcriptomics and proteomics studies on bromobenzene-mediated hepatotoxicity in the rat are discussed. Finally, an example is shown in which gene expression profiling together with conventional biochemistry led to the discovery of novel markers for the hepatic effects of the food additives butylated hydroxytoluene, curcumin, propyl gallate and thiabendazole. © 2005 Elsevier Inc. All rights reserved.
Subject
Toxicology Nutrition
Physiological Sciences
In vitro
In vivo
Liver
Toxicogenomics
bromobenzene
butylcresol
chemical compound
curcumin
food additive
gallic acid propyl ester
proteome
tiabendazole
transcriptome
biochemistry
bioinformatics
comparative toxicology
conference paper
disease marker
DNA fingerprinting
gene expression profiling
genetic procedures
genetic toxicology
genomics
in vitro study
in vivo study
laboratory
liver toxicity
metabolomics
molecular biology
nonhuman
prediction
proteomics
rat
technology
toxicological parameters
transcriptomics
Animals
Food Additives
Genomics
Humans
Liver
Toxicology
To reference this document use:
http://resolver.tudelft.nl/uuid:c1ec68db-a727-440c-9102-5a83c0592f2c
DOI
https://doi.org/10.1016/j.taap.2005.01.050
TNO identifier
238674
ISSN
0041-008X
Source
Toxicology and Applied Pharmacology, 207 (2 SUPPL.)
Document type
article