Title
Localization in situ of costimulatory molecules and cytokines in B-cell non-Hodgkin's lymphoma
Author
Vyth-Dreese, F.A.
Boot, H.
Dellemijn, T.A.M.
Majoor, D.M.
Oomen, L.C.J.M.
Laman, J.D.
van Meurs, M.
de Weger, R.A.
de Jong, D.
TNO Preventie en Gezondheid
Publication year
1998
Abstract
Costimulatory molecules are essential in cognate interactions between T and B lymphocytes. To study the prerequisites of functional interactions between malignant B cells and intermingled T cells in B-cell non-Hodgkin's lymphomas (B-NHL), we examined the expression of CD40, CD80 and CD86 and their ligands CD40 ligand (CD40L, CD154), CD28 and CTLA4 (CD152) using immunohistochemistry and confocal laser scanning microscopy. Almost all mucosa-associated lymphoid tissue (MALT) NHL were positive for CD40 and CD80 and in nine out of 14 cases were positive for CD86. The majority of follicle centre cell lymphomas (FCCL) expressed CD40, but were heterogeneous in their expression of CD80 and CD86. Most diffuse large cell lymphomas (DLCL) were CD80+, but lacked expression of CD86. These patterns reflect the differences in phenotype of normal marginal-zone B cells (as counterparts of MALT NHL) and germinal centre cells (as counterparts of FCCL and DLCL). Counter- receptors on T cells were detectable in 13 of 14 MALT NHL, 12 of 16 FCCL but only occasionally in DLCL (three of 12 cases). A subgroup of FCCL was identified with T-cell expression of CD40L, CD28 and CTLA4 simultaneously with strong expression of CD40 and CD86 on the tumour B cells. These results indicate that MALT NHL and a subset of FCCL are most optimally equipped for functional interactions with T cells. This may be supported by the demonstration of cytokine production mainly in T cells - in MALT NHL [interleukin-2 (IL-2), interferon-γ (IFN-γ), IL-10] and FCCL (IL-2, IFN- γ) and to a lesser extent in DLCL.
Subject
Health
Antigens, CD
Antigens, CD40
Antigens, CD80
Antigens, CD86
Antigens, Differentiation
B-Lymphocytes
CD40 Ligand
Cytokines
Humans
Immunoconjugates
Immunohistochemistry
Ligands
Lymphoma, B-Cell
Lymphoma, Large-Cell, Diffuse
Lymphoma, Mucosa-Associated Lymphoid Tissue
Membrane Glycoproteins
Microscopy, Confocal
T-Lymphocytes
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DOI
https://doi.org/10.1046/j.1365-2567.1998.00550.x
TNO identifier
234579
ISSN
0019-2805
Source
Immunology, 94 (4), 580-586
Document type
article