Print Email Facebook Twitter A dietary mixture containing fish oil, resveratrol, lycopene, catechins, and vitamins E and C reduces atherosclerosis in transgenic mice Title A dietary mixture containing fish oil, resveratrol, lycopene, catechins, and vitamins E and C reduces atherosclerosis in transgenic mice Author Verschuren, L. Wielinga, P.Y. van Duyvenvoorde, W. Tijani, S. Toet, K. van Ommen, B. Kooistra, T. Kleemann, R. Publication year 2011 Abstract Chronic inflammation and proatherogenic lipids are important risk factors of cardiovascular disease (CVD). Specific dietary constituents such as polyphenols and fish oils may improve cardiovascular risk factors and may have a beneficial effect on disease outcomes. We hypothesized that the intake of an antiinflammatory dietary mixture (AIDM) containing resveratrol, lycopene, catechin, vitamins E and C, and fish oil would reduce inflammatory risk factors, proatherogenic lipids, and endpoint atherosclerosis. AIDM was evaluated in an inflammation model, male human C-reactive protein (CRP) transgenic mice, and an atherosclerosis model, female ApoE*3Leiden transgenic mice. Two groups of male human-CRP transgenic mice were fed AIDM [0.567% (wt:wt) powder and 0.933% (wt:wt oil)] or placebo for 6 wk. The effects of AIDM on basal and IL-1ß-stimulated CRP expression were investigated. AIDM reduced cytokine-induced human CRP and fibrinogen expression in human-CRP transgenic mice. In the atherosclerosis study, 2 groups of female ApoE*3Leiden transgenic mice were fed an atherogenic diet supplemented with AIDM [0.567% (wt:wt) powder and 0.933% (wt:wt oil)] or placebo for 16 wk. AIDM strongly reduced plasma cholesterol, TG, and serum amyloid A concentrations compared with placebo. Importantly, long-term treatment of ApoE*3Leiden mice with AIDM markedly reduced the development of atherosclerosis by 96% compared with placebo. The effect on atherosclerosis was paralleled by a reduced expression of the vascular inflammation markers and adhesion molecules inter-cellular adhesion molecule-1 and E-selectin. Dietary supplementation of AIDM improves lipid and inflammatory risk factors of CVD and strongly reduces atherosclerotic lesion development in female transgenic mice. © 2011 American Society for Nutrition. Subject LifeMSB - Microbiology and Systems Biology MHR - Metabolic Health ResearchEELS - Earth, Environmental and Life SciencesNutritionalpha tocopherolapolipoprotein E3ascorbic acidbiological markerC reactive proteincatechincell adhesion moleculecholesterolcytokinedocosahexaenoic acidendothelial leukocyte adhesion molecule 1epigallocatechin gallatefibrinogenfish oilgreen tea extracticosapentaenoic acidintercellular adhesion molecule 1interleukin 1betalipidlycopeneomega 3 700placebopolyunsaturated fatty acidresveratrolserum amyloid Atriacylglycerolunclassified druganimal experimentanimal modelantiinflammatory dietary mixturearticleatherogenic dietatherosclerosischolesterol blood levelcontrolled studydiet supplementationdietary intakedrug efficacydrug megadosefeedingfemaleinflammationlong term caremalemousenonhumanpowderprotein blood levelprotein expressionrisk factorrisk reductiontransgenic animaltreatment durationtreatment outcometriacylglycerol blood levelvasculitisomega 3 700Solgar Vitamin and Herb To reference this document use: http://resolver.tudelft.nl/uuid:b4a1bb12-02cd-4c01-a07d-7c047fd51bb1 DOI https://doi.org/10.3945/jn.110.133751 TNO identifier 429731 ISSN 0022-3166 Source Journal of Nutrition, 141 (5), 863-869 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.