Title
Induction of type II collagen-specific antibody production in blood lymphocyte cultures of Rhesus monkeys (Macaca mulatta) with collagen-induced arthritis using the immobilized native antigen
Author
't Hart, B.A.
Botman, C.A.D.
Bakker, N.P.M.
Publication year
1991
Abstract
Peripheral blood mononuclear cells (PBMC) from Rhesus monkeys previously immunized with bovine type II collagen to induce arthritis were cultured with the same antigen. Because the native protein is poorly soluble in culture medium a heating step is often used. The antigen in this form induced PBMC proliferation, but epitopes for the induction of antibody production and arthritis were lost. To keep the native protein intact, it was coated on affigel beads. With the immobilized antigen specific antibody production could be induced.
Subject
Antibody production
Peripheral blood mononuclear cells
Type II collagen
collagen type 2
aged
animal cell
animal model
antibody production
arthritis
article
controlled study
immunization
monkey
nonhuman
priority journal
Animal
Antibody Specificity
Arthritis
Autoantibodies
Collagen
Disease Models, Animal
Heat
Immunization
Lymphocytes
Macaca mulatta
Sepharose
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http://resolver.tudelft.nl/uuid:b3729fd3-a15c-42c7-80a9-5c2286385a98
TNO identifier
231453
ISSN
0009-9104
Source
Clinical and Experimental Immunology, 83 (83), 375-378
Bibliographical note
Correspondence Address: 'T Hart, B.A.; Dept. Chronic/Infectious Dis., ITRI-TNO Institute, PO Box 5815, 2280 HV Rijswijk, Netherlands Chemicals/CAS: Affi-Gel 15, 79920-18-6; Autoantibodies; Collagen, 9007-34-5; Sepharose, 9012-36-6
Document type
article