Induction of type II collagen-specific antibody production in blood lymphocyte cultures of Rhesus monkeys (Macaca mulatta) with collagen-induced arthritis using the immobilized native antigen
't Hart, B.A.
Peripheral blood mononuclear cells (PBMC) from Rhesus monkeys previously immunized with bovine type II collagen to induce arthritis were cultured with the same antigen. Because the native protein is poorly soluble in culture medium a heating step is often used. The antigen in this form induced PBMC proliferation, but epitopes for the induction of antibody production and arthritis were lost. To keep the native protein intact, it was coated on affigel beads. With the immobilized antigen specific antibody production could be induced.
To reference this document use:
Peripheral blood mononuclear cells
Type II collagen
collagen type 2
Disease Models, Animal
Clinical and Experimental Immunology, 83 (83), 375-378
Correspondence Address: 'T Hart, B.A.; Dept. Chronic/Infectious Dis., ITRI-TNO Institute, PO Box 5815, 2280 HV Rijswijk, Netherlands Chemicals/CAS: Affi-Gel 15, 79920-18-6; Autoantibodies; Collagen, 9007-34-5; Sepharose, 9012-36-6