Title
Hepatic glucose production is more sensitive to insulin-mediated inhibition than hepatic VLDL-triglyceride production
Author
den Boer, M.A.M.
Voshol, P.J.
Kuipers, F.
Romijn, J.A.
Havekes, L.M.
TNO Kwaliteit van Leven
Publication year
2006
Abstract
Insulin is an important inhibitor of both hepatic glucose output and hepatic VLDL-triglyceride (VLDL-TG) production. We investigated whether both processes are equally sensitive to insulin-mediated inhibition. To test this, we used euglycemic clamp studies with four increasing plasma concentrations of insulin in wild-type C57Bl/6 mice. By extrapolation, we estimated that half-maximal inhibition of hepatic glucose output and hepatic VLDL-TG production by insulin were obtained at plasma insulin levels of ∼3.6 and ∼6.8 ng/ml, respectively. In the same experiments, we measured that half-maximal decrease of plasma free fatty acid levels and half-maximal stimulation of peripheral glucose uptake were reached at plasma insulin levels of ∼3.0 and ∼6.0 ng/ml, respectively. We conclude that, compared with insulin sensitivity of hepatic glucose output, peripheral glucose uptake and hepatic VLDL-TG production are less sensitive to insulin. Copyright © 2006 the American Physiological Society. Chemicals / CAS: insulin, 9004-10-8; Blood Glucose; Fatty Acids, Nonesterified; Glucose, 50-99-7; Hypoglycemic Agents; Insulin, 11061-68-0; Lipoproteins, VLDL; Triglycerides
Subject
Biology
Biomedical Research
Euglycemic hyperinsulinemic clamp
Fatty acid
Very-low-density lipoprotein
Insulin
Adipose tissue
Animal experiment
Aanimal model
Controlled study
Drug effect
Drug inhibition
Fatty acid blood level
Gluconeogenesis
Glucose infusion
Glucose transport
Hyperinsulinemia
Insulin blood level
Insulin resistance
Insulin sensitivity
Lipolysis
Liver function
Mouse
Nonhuman
Animals
Blood Glucose
Dose-Response Relationship, Drug
Fatty Acids, Nonesterified
Glucose
Glucose Clamp Technique
Hypoglycemic Agents
Insulin
Kinetics
Lipoproteins, VLDL
Liver
Male
Mice
Mice, Inbred C57BL
Triglycerides
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DOI
https://doi.org/10.1152/ajpendo.00188.2006
TNO identifier
239769
ISSN
0193-1849
Source
American Journal of Physiology - Endocrinology and Metabolism, 291 (6)
Document type
article