Title
Performance and population analysis of a non-sterile trickle bed reactor inoculated with caldicellulosiruptor saccharolyticus, a thermophilic hydrogen producer
Author
van Groenestijn, J.W.
Geelhoed, J.S.
Goorissen, H.P.
Meesters, K.P.M.
Stams, A.J.M.
Claassen, P.A.M.
TNO Kwaliteit van Leven
Publication year
2009
Abstract
Non-axenic operation of a 400 L trickle bed reactor inoculated with the thermophile Caldicellulosiruptor saccharolyticus, yielded 2.8 molH 2mol hexose converted. The reactor was fed with a complex medium with sucrose as the main substrate, continuously flushed with nitrogen gas, and operated at 73°C. The volumetric productivity was 22 mmolH2(L filterbed h). Acetic acid and lactic acid were the main by-products in the liquid phase. Production of lactic acid occurred when hydrogen partial pressure was elevated above 2% and during suboptimal fermentation conditions that also resulted in the presence of monoand disaccharides in the effluent. Methane production was negligible. The microbial community was analyzed at two different time points during operation. Initially, other species related to members of the genera Thermoanaerobacterium and Caldicellulosiruptor were present in the reactor. However, these were out-competed by C. saccharolyticus during a period when sucrose was completely used and no saccharides were discharged with the effluent. In general, the use of pure cultures in non-sterile industrial applications is known to be less useful because of contamination. However, our results show that the applied fermentation conditions resulted in a culture of a single dominant organism with excellent hydrogen production characteristics. © 2008 Wiley Periodicals, Inc.
Subject
Biology
Biotechnology
Hydrogen
Thermophiles
Trickle bed reactor
Caldicellulosiruptor saccharolyticus
Complex mediums
Hydrogen partial pressures
Liquid-phase
Methane productions
Microbial communities
Nitrogen gas
Population analysis
Pure cultures
Thermoanaerobacterium
Thermophiles
Time points
Trickle bed reactor
Volumetric productivities
Biochemical engineering
Body fluids
Chemical reactors
Effluents
Fermentation
Gas producers
Hydrogen
Industrial applications
Lactic acid
Methane
Organic acids
Polysaccharides
Sugar (sucrose)
Hydrogen production
acetic acid
disaccharide
hexose
hydrogen
lactic acid
methane
monosaccharide
nitrogen
sucrose
alcohol production
article
bacterial strain
bacterium isolation
Caldicellulosiruptor saccharolyticus
controlled study
fermentation
Gram positive bacterium
inoculation
microbial community
microbial diversity
New Zealand
nonhuman
pressure
reactor
temperature sensitivity
Thermoanaerobacterium
Acetic Acid
Biodiversity
Bioreactors
Culture Media
DNA, Bacterial
DNA, Ribosomal
Glucose
Gram-Positive Bacteria
Hydrogen
Lactic Acid
Methane
Molecular Sequence Data
RNA, Ribosomal, 16S
Sequence Analysis, DNA
Sucrose
Temperature
Thermoanaerobacterium
Caldicellulosiruptor
Caldicellulosiruptor saccharolyticus
Thermoanaerobacterium
To reference this document use:
http://resolver.tudelft.nl/uuid:ae8de0fc-b18a-4a30-82f3-1313ae6afb19
DOI
https://doi.org/10.1002/bit.22185
TNO identifier
241473
ISSN
0006-3592
Source
Biotechnology and Bioengineering, 102 (5), 1361-1367
Document type
article