Title
Role of amino acids in rheumatoid arthritis studied by metabolomics
Author
He, M.
Harms, A.C.
van Wijk, E.
Wang, M.
Berger, R.
Koval. S.,
Hankemeier, T.
van der Greef, J.
Publication year
2019
Abstract
Background. Rheumatoid arthritis (RA) is a complex, chronic autoimmune disease characterized by various inflammatory symptoms, including joint swelling, joint pain, and both structural and functional joint damage. The most commonly used animal model for studying RA is mice with collagen‐induced arthritis (CIA); the wide use of this model is due primarily to many similarities with RA in human patients. Metabolomics is used increasingly in biological studies for diagnosing disease and for predicting and evaluating drug interventions, as a large number of disease‐associated metabolites can be analyzed and interpreted from a biological perspective. Aim. To profile free amino acids and their biogenic metabolites in CIA mice plasma. Method. Ultra‐high‐performance liquid chromatography/tandem mass spectrometry coupled with multiple reaction monitoring (MRM) was used for metabolomics study. Results. Profile of 45 amine metabolites, including free amino acids and their biogenic metabolites in plasma was obtained from CIA mice. We found that the plasma levels of 20 amine metabolites were significantly decreased in the CIA group. Conclusion. The results suggest that a disordered amine response is linked to RA‐associated muscle wasting and energy expenditure.
Subject
Amine metabolites
Collagen-induced arthritis
Mouse model
Systems biology
Biomedical Innovation
Healthy Living
Life
MSB - Microbiology and Systems Biology
ELSS - Earth, Life and Social Sciences
To reference this document use:
http://resolver.tudelft.nl/uuid:a75bba87-4c85-4428-b4e1-31e5f8bcf61d
DOI
https://doi.org/10.1111/1756-185x.13062
TNO identifier
867478
Source
International Journal of Rheumatic Diseases, 22 (22), 38-46
Document type
article