Title
Animal products and K-ras codon 12 and 13 mutations in colon carcinomas
Author
Kampman, E.
Voskuil, D.W.
van Kraats, A.A.
Balder, H.F.
van Muijen, G.N.P.
Goldbohm, R.A.
van 't Veer, P.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
2000
Abstract
K-ras gene mutations (codons 12 and 13) were determined by PCR-based mutant allele-specific amplification (MASA) in tumour tissue of 185 colon cancer patients: 36% harboured mutations, of which 82% were located in codon 12. High intakes of animal protein, calcium and poultry were differently associated with codon 12 and 13 mutations: odds ratios (OR) and 95% confidence intervals (95% CI) for codon 12 versus codon 13 were 9.0 (2.0-42), 4.1 (1.4-12) and 15 (1.4-160), respectively. In case-control comparisons, high intakes of animal protein and calcium were positively associated with colon tumours harbouring codon 12 mutations [for animal protein per 17 g, OR (95% CI) = 1.5 (1.0-2.1); for calcium per 459 mg, 1.2 (0.9-1.6)], while inverse associations were observed for tumours with K-ras mutations in codon 13 [for animal protein 0.4 (0.2-1.0); for calcium 0.6 (0.3-1.2)]. Transition and transversion mutations were not differently associated with these dietary factors. These data suggest a different dietary aetiology of colon tumours harbouring K-ras codon 12 and 13 mutations Chemicals/CAS: Calcium, Dietary; Codon; Dietary Fats; Dietary Proteins; DNA Adducts; DNA, Neoplasm
Subject
Nutrition
Calcium
Protein
Adult
Aged
Allele
Animal
Cancer risk
Case control study
Codon
Colon cancer
Controlled study
Diet
Female
Gene mutation
Human
Major clinical study
Male
Meat
Oncogene K ras
Polymerase chain reaction
Poultry
Priority journal
Statistical analysis
Animals
Calcium, Dietary
Carcinoma
Case-Control Studies
Codon
Colonic Neoplasms
Dairy Products
Dietary Fats
Dietary Proteins
DNA Adducts
DNA Mutational Analysis
DNA, Neoplasm
Female
Fishes
Food Habits
Genes, ras
Humans
Male
Meat
Middle Aged
Mutation
Netherlands
Odds Ratio
Polymerase Chain Reaction
Poultry
Questionnaires
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TNO identifier
41374
ISSN
0143-3334
Source
Carcinogenesis, 21 (2), 307-309
Document type
article