Title
Do aberrant crypt foci have predictive value for the occurrence of colorectal tumours? Potential of gene expression profiling in tumours
Author
Wijnands, M.V.W.
van Erk, M.J.
Doornbos, R.P.
Krul, C.A.M.
Woutersen, R.A.
Publication year
2004
Abstract
The effects of different dietary compounds on the formation of aberrant crypt foci (ACF) and colorectal tumours and on the expression of a selection of genes were studied in rats. Azoxymethane-treated male F344 rats were fed either a control diet or a diet containing 10% wheat bran (WB), 0.2% curcumin (CUR), 4% rutin (RUT) or 0.04% benzyl isothiocyanate (BIT) for 8 months. ACF were counted after 7, 15 and 26 weeks. Tumours were scored after 26 weeks and 8 months. We found that the WB and CUR diets inhibited the development of colorectal tumours. In contrast, the RUT and BIT diets rather enhanced (although not statistically significantly) colorectal carcinogenesis. In addition, the various compounds caused different effects on the development of ACF. In most cases the number or size of ACF was not predictive for the ultimate tumour yield. The expression of some tumour-related genes was significantly different in tumours from the control group as compared to tumours from the treated groups. It was concluded that WB and CUR, as opposed to RUT and BIT, protects against colorectal cancer and that ACF are unsuitable as biomarker for colorectal cancer. Effects of the different dietary compounds on metalloproteinase 1 (TIMP-1) expression correlated well with the effects of the dietary compounds on the ultimate tumour yield. © 2004 Elsevier Ltd. All rights reserved. Chemicals / CAS: benzyl isothiocyanate, 622-78-6; curcumin, 458-37-7; rutoside, 153-18-4, 22519-99-9; Anticarcinogenic Agents; DNA Primers; DNA, Complementary; RNA, Neoplasm; Tissue Inhibitor of Metalloproteinase-1; Tumor Markers, Biological
Subject
Biology
Analytical research
AC, aberrant crypt
ACF, aberrant crypt focus
AIN, American Institute of Nutrition
ANOVA, analysis of variance
AOM, azoxymethane
BIT, benzyl isothiocyanate
CA2, carbonic anhydrase II
CDK4, cyclin-dependent kinase 4
cDNA, complementary DNA
benzyl isothiocyanate
curcumin
rutoside
animal experiment
animal model
animal tissue
antineoplastic activity
article
carcinogenesis
colorectal tumor
controlled study
correlation analysis
crypt cell
dietary intake
disease marker
drug effect
gene expression profiling
intestine crypt
male
nonhuman
oncogene
prediction
protein expression
rat
scoring system
statistical significance
time series analysis
wheat bran
Animals
Anticarcinogenic Agents
Body Weight
Colorectal Neoplasms
Diet
DNA Primers
DNA, Complementary
Eating
Energy Metabolism
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Intestinal Mucosa
Male
Predictive Value of Tests
Rats
Rats, Inbred F344
Reverse Transcriptase Polymerase Chain Reaction
RNA, Neoplasm
Survival Analysis
Tissue Inhibitor of Metalloproteinase-1
Tumor Markers, Biological
Triticum aestivum
To reference this document use:
http://resolver.tudelft.nl/uuid:996e316b-c942-4038-90b6-ff572ed5eb48
DOI
https://doi.org/10.1016/j.fct.2004.05.008
TNO identifier
238028
ISSN
0278-6915
Source
Food and Chemical Toxicology, 42 (10), 1629-1639
Document type
article