Print Email Facebook Twitter A generic model for glucose production from various cellulose sources by a commercial cellulase complex Title A generic model for glucose production from various cellulose sources by a commercial cellulase complex Author Drissen, R.E.T. Maas, R.H.W. van der Maarel, M.J.E.C. Kabel, M.A. Schols, H.A. Tramper, J. Beeftink, H.H. TNO Kwaliteit van Leven Publication year 2007 Abstract The kinetics of cellulose hydrolysis by commercially available Cellubrix were described mathematically, with Avicel and wheat straw as substrates. It was demonstrated that hydrolysis could be described by three reactions: direct glucose formation and indirect glucose formation via cellobiose. Hydrolysis did not involve any soluble oligomers apart from low amounts of cellobiose. Phenomena included in the mathematical model were substrate limitation, adsorption of enzyme onto substrate, glucose inhibition, temperature dependency of reaction rates, and thermal enzyme inactivation. In addition, substrate heterogeneity was described by a recalcitrance constant. Model parameters refer to both enzyme characteristics and substrate-specific characteristics. Quantitative model development was carried out on the basis of Avicel hydrolysis. In order to describe wheat straw hydrolysis, wheat straw specific parameter values were measured. Updating the pertinent parameters for wheat straw yielded a satisfactory description of wheat straw hydrolysis, thus underlining the generic potential of the model. Chemicals / CAS: cellobiose, 16462-44-5, 528-50-7; cellulose, 61991-22-8, 68073-05-2, 9004-34-6; glucose, 50-99-7, 84778-64-3; microcrystalline cellulose, 39394-43-9, 51395-75-6 Subject HealthCelluloseGlucoseModelAdsorptionCelluloseEnzyme inhibitionHydrolysisMathematical modelsOligomersReaction ratesCellobioseEnzyme inactivationGlucose formationGlucoseTriticum aestivum To reference this document use: http://resolver.tudelft.nl/uuid:987a1681-94c1-4a5c-966b-dbcc9fbbc5a1 DOI https://doi.org/10.1080/10242420701510668 TNO identifier 240261 ISSN 1024-2422 Source Biocatalysis and Biotransformation, 25 (6), 419-429 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.