Title
The integration of single fiber reflectance (SFR) spectroscopy during endoscopic ultrasound-guided fine needle aspirations (EUS-FNA)in pancreatic masses: A feasibility study
Author
Stegehuis, P.L.
Boogerd, L.S.F.
Inderson, A.
Veenendaal, R.A.
Bonsing, B.A.
Amelink, A.
Vahrmeijer, A.L.
Dijkstra, J.
Robinson, D.J.
Contributor
Tearney, G.J. (editor)
Wang, T.D. (editor)
Suter, M.J. (editor)
Brenner, M. (editor)
Lam, S. (editor)
Publication year
2016
Abstract
EUS-FNA can be used for pathological confirmation of a suspicious pancreatic mass. However, performance depends on an on-site cytologist and time between punction and final pathology results can be long. SFR spectroscopy is capable of extracting biologically relevant parameters (e.g. oxygenation and blood volume) in real-time from a very small tissue volume at difficult locations. In this study we determined feasibility of the integration of SFR spectroscopy during EUSFNA procedures in pancreatic masses. Patients with benign and malignant pancreatic masses who were scheduled for an EUS-FNA were included. The working guide wire inside the 19 gauge endoscopic biopsy needle was removed and the sterile single fiber (300 μm core and 700 μm outer diameter, wide-angle beam, NA 0.22) inserted through the needle. Spectroscopy measurements in the visiblenear infrared wavelength region (400-900 nm) and autofluorescence measurements (excitation at 405 nm) were taken three times, and subsequently cytology was obtained. Wavelength dependent optical properties were compared to cytology results. We took measurements in 13 patients with corresponding cytology results (including mucinous tumor, ductal adenocarcinoma, neuroendocrine tumor, and pancreatitis). In this paper we show the first analyzed results comparing normal pancreatic tissue with cancerous tissue in the same patient. We found a large difference in blood volume fraction, and blood oxygenation was higher in normal tissue. Integration of SFR spectroscopy is feasible in EUS-FNA procedures, the workflow hardly requires changes and it takes little time. The first results differentiating normal from tumor tissue are promising.
Subject
Nano Technology
OPT - Optics
TS - Technical Sciences
High Tech Systems & Materials
Electronics
Industrial Innovation
Endoscopy
Single fiber reflectance spectroscopy
Cytology
Hemodynamics
Integration
Medicine
Optical properties
Oxygenation
Ultrasonics
Ductal adenocarcinomas
Endoscopic ultrasounds
Fine-needle aspirations
Neuroendocrine tumors
Pancreatic tumors
Reflectance spectroscopy
Spectroscopy measurements
Wavelength-dependent optical properties
Tissue engineering
To reference this document use:
http://resolver.tudelft.nl/uuid:85ad0ee5-7731-4b78-9b8f-dedfa72dbf6f
TNO identifier
537438
Publisher
SPIE
ISBN
9781628419252
ISSN
1605-7422
Source
Endoscopic Microscopy XI and Optical Techniques in Pulmonary Medicine III, 9691
Series
Proceedings of SPIE
Article number
96910J
Document type
conference paper