Print Email Facebook Twitter Screening and characterization of Lactobacillus strains producing large amounts of exopolysaccharides Title Screening and characterization of Lactobacillus strains producing large amounts of exopolysaccharides Author van Geel-Schutten, G.H. Flesch, F. ten Brink, B. Smith, M.R. Dijkhuizen, L. Centraal Instituut voor Voedingsonderzoek TNO Publication year 1998 Abstract A total of 182 Lactobacillus strains were screened for production of extracellular polysaccharides (EPS) by a new method: growth in liquid media with high sugar concentrations. Sixty EPS-positive strains were identified; 17 strains produced more than 100 mg/l soluble EPS. Sucrose was an excellent substrate for abundant EPS synthesis. The ability to produce glucans appears to be widespread in the genus Lactobacillus. The monosaccharide composition of EPS produced by Lactobacillus reuteri strain LB 121 varied with the growth conditions (solid compared to liquid medium) and the sugar substrates (sucrose or raffinose) supplied in the medium. Strain LB 121 produced both a glucan and a fructan on sucrose, but only a fructan on raffinose. This is the first report of fructan production by a Lactobacillus species. EPS production increased with increasing sucrose concentrations and involved extracellular sucrase-type enzymes. Subject NutritionFructanPolysaccharideRaffinoseSucroseBacterial metabolismCarbohydrate synthesisConcentration responseLactobacillus reuteriNonhumanStrain differenceLactobacillus reuteri To reference this document use: http://resolver.tudelft.nl/uuid:84518bdb-3d16-4eff-8fab-76496cca5485 DOI https://doi.org/10.1007/s002530051353 TNO identifier 234708 ISSN 0175-7598 Source Applied Microbiology and Biotechnology, 50 (6), 697-703 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.