Title
Molecular assessment of bacterial vaginosis by Lactobacillus abundance and species diversity
Author
Dols, J.A.M.
Molenaar, D.
van der Helm, J.J.
Caspers, M.P.M.
de Kat Angelino-Bart, A.
Schuren, F.H.J.
Speksnijder, A.G.C.L.
Westerhoff, H.V.
Richardus, J.H.
Boon, M.E.
Reid, G.
de Vries, H.J.C.
Kort, R.
Publication year
2016
Abstract
Background: To date, women are most often diagnosed with bacterial vaginosis (BV) using microscopy based Nugent scoring or Amsel criteria. However, the accuracy is less than optimal. The aim of the present study was to confirm the identity of known BV-associated composition profiles and evaluate indicators for BV using three molecular methods. Methods: Evaluation of indicators for BV was carried out by 16S rRNA amplicon sequencing of the V5-V7 region, a tailor-made 16S rRNA oligonucleotide-based microarray, and a PCR-based profiling technique termed IS-profiling, which is based on fragment variability of the 16S-23S rRNA intergenic spacer region. An inventory of vaginal bacterial species was obtained from 40 females attending a Dutch sexually transmitted infection outpatient clinic, of which 20 diagnosed with BV (Nugent score 7–10), and 20 BV negative (Nugent score 0–3).
Subject
Life
MSB - Microbiology and Systems Biology
ELSS - Earth, Life and Social Sciences
Food and Nutrition
Nutrition
Healthy Living
Vaginal microbiota
Bacterial vaginosis
Nucleotide-based microarrays
16S rRNA amplicon sequencing
IS-profiling, Lactobacillus crispatus
Lactobacillus iners
Gardnerella vaginalis
Gini-Simpson index
To reference this document use:
http://resolver.tudelft.nl/uuid:8370a4ae-e45c-4caa-ac71-29e5fe8dea09
DOI
https://doi.org/10.1186/s12879-016-1513-3
TNO identifier
534811
Source
BMC Infectious Diseases, 16
Article number
180
Document type
article