Title
Analyses of human colonic mucus obtained by an in vivo sampling technique
Author
Hamer, H.M.
Jonkers, D.M.A.E.
Loof, A.
van Houtvin, S.A.L.W.
Troost, F.J.
Venema, K.
Kodde, A.
Koek, G.H.
Schipper, R.G.
van Heerde, W.L.
Brummer, R.J.
TNO Kwaliteit van Leven
Publication year
2009
Abstract
Background: The mucus layer is an important dynamic component of the epithelial barrier. It contains mucin glycoproteins and other compounds secreted by the intestinal epithelium, such as secretory IgA. However, a standardized in vivo sampling technique of mucus in humans is not yet available. Aim: To assess the validity and feasibility of mucin and protein determinations in human colonic mucus collected under physiological conditions. Subjects and methods: Triplicate colonic mucus samples were collected in 11 healthy volunteers using cytology brushes during sigmoidoscopy. As an indication of the quantity of collected mucus, total protein and mucin concentrations were determined by measuring oligosaccharide equivalents and monosaccharides. Also secretory IgA and sialic acid concentrations were determined and proteomic analysis was performed using surface enhanced laser desorption/ionization-time of flight-mass spectrometry. Results: Mean values of secretory IgA and sialic acid corrected for the amount of mucus ranged from 0.16 to 1.81 g secretory IgA/mmol oligosaccharide equivalents and from 12.6 to 48.6 g sialic acid/mmol oligosaccharide equivalents. Proteomic analysis of mucus is feasible and cluster analysis showed subject specific profiles. Conclusion: Using cytology brushes, human colonic mucus can be sampled and under physiological conditions. These samples could give information on the composition and quality of the mucus layer. © 2009 Editrice Gastroenterologica Italiana S.r.l.
Subject
Biology Health
Biomedical Research
Colon
Mucus
Proteomics
Secretory IgA
fucose
galactosamine
galactose
glucosamine
glycoprotein
Immunoglobulin A
Monosaccharide
Mucin
Oligosaccharide
Sialic acid
Adult
Anion exchange chromatography
Colon mucosa
Colon mucus
Concentration (parameters)
Cytology
Female
Human
Human tissue
In vivo study
Intestine epithelium
Male
Mucus
Normal human
Priority journal
Protein analysis
Proteomics
Sampling
Sigmoidoscopy
Surface enhanced laser desorption ionization time of flight mass spectrometry
Volunteer
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http://resolver.tudelft.nl/uuid:7f5c279c-6758-4be3-be67-7b40e55bdeb5
DOI
https://doi.org/10.1016/j.dld.2008.12.100
TNO identifier
241666
ISSN
1590-8658
Source
Digestive and Liver Disease, 41 (8), 559-564
Document type
article