Title
Expression of agsA, one of five 1,3-α-d-glucan synthase-encoding genes in Aspergillus niger, is induced in response to cell wall stress
Author
Damveld, R.A.
van Kuyk, P.A.
Arentshorst, M.
Klis, F.M.
van den Hondel, C.A.M.J.J.
Ram, A.F.J.
TNO Kwaliteit van Leven TNO Voeding
Publication year
2005
Abstract
1,3-α-d-Glucan is an important component of the cell wall of filamentous fungi. We have identified a family of five 1,3-α-d-glucan synthase-encoding genes in Aspergillus niger. The agsA gene was sequenced and the predicted protein sequence indicated that the overall domain structure of 1,3-α-d-glucan synthases is conserved in fungi. Using RT-PCR and Northern blot analysis, we found that expression of the agsA gene and to a lesser extent also of agsE were induced in the presence of the cell wall stress-inducing compounds such as Calcofluor White (CFW), SDS, and caspofungin. Loss of agsA function did not result in an apparent phenotype under normal growth conditions but rendered the cells more sensitive to CFW. The induction of 1,3-α-d-glucan synthase-encoding genes in response to cell wall stress was not limited to A. niger, but was also observed in Penicillium chrysogenum. We propose that this response to cell wall stress commonly occurs in filamentous fungi. © 2004 Elsevier Inc. All rights reserved.
Subject
Biology
Biotechnology
1,3-α-d-Glucan
Calcofluor White
Cell wall remodelling
Cell wall stress
Duplex RT-PCR
Signalling
Caspofungin
Glucan synthase
Amino acid sequence
Animal cell
Aspergillus niger
Cell wall
Fungus
Gene expression
Nonhuman
Northern blotting
Nucleotide sequence
Phenotype
Priority journal
Reverse transcription polymerase chain reaction
Stress
Amino Acid Sequence
Aspergillus niger
Benzenesulfonates
Cell Wall
Gene Deletion
Gene Expression
Gene Expression Regulation, Fungal
Genes, Fungal
Glucosyltransferases
Molecular Sequence Data
Phylogeny
Sequence Alignment
Aspergillus
Aspergillus niger
Fungi
Penicillium
Penicillium chrysogenum
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http://resolver.tudelft.nl/uuid:78cf9d6d-6e63-4fb9-b4b4-9e7330790c19
DOI
https://doi.org/10.1016/j.fgb.2004.11.006
TNO identifier
238323
ISSN
1087-1845
Source
Fungal Genetics and Biology, 42 (2), 165-177
Document type
article