Title
Determination of polar organophosphorus pesticides in vegetables and fruits using liquid chromatography with tandem mass spectrometry: Selection of extraction solvent
Author
Mol, H.G.J.
van Dam, R.C.J.
Steijger, O.M.
TNO Voeding
Publication year
2003
Abstract
A method based on liquid chromatography (LC)-mass spectrometry (MS)/MS was developed for sensitive determination of a number of less gas chromatography (GC)-amenable organophosphorus pesticides (OPs; acephate, methamidophos, monocrotophos, omethoate, oxydemeton-methyl and vamidothion) in cabbage and grapes. For extraction, several solvents were evaluated with respect to the possibility of direct injection, matrix-induced suppression or enhancement of response, and extraction efficiency. Overall, ethyl acetate was the most favourable solvent for extraction, although a solvent switch was required. For some pesticide/matrix combinations, reconstitution of the residue after evaporation required special attention. Extracts were analysed on a C18 column with polar endcapping. The pesticides were ionised using atmospheric pressure chemical ionisation on a tandem mass spectrometer in multiple reaction monitoring mode. The final method is straightforward and involves extraction with ethyl acetate and a solvent switch to 0.1% acetic acid/water without further cleanup. The method was validated at the 0.01 and 0.5mg/kg level, for both cabbage and grapes. Recoveries were between 80 and 101% with R.S.D.<11% (n=5). The limit of quantification was 0.01mg/kg and limits of detection were between 0.001 and 0.004mg/kg. © 2003 Elsevier B.V. All rights reserved.
Subject
Packaging
Analytical research
Acephate
Fruits
Methamidophos
Monocrotophos
Omethoate
Organophosphorus compounds
Oxydemeton-methyl
Pesticides
Vamidothion
Vegetables
Fruits
Ionization
Mass spectrometry
Pesticides
Solvent extraction
Extraction efficiency
Liquid chromatography
acephate
acetic acid ethyl ester
methamidophos
monocrotophos
omethoate
organophosphate pesticide
oxydemeton methyl
solvent
unclassified drug
vamidothion
water
cabbage
conference paper
evaporation
extraction
fruit
grape
ionization
liquid chromatography
priority journal
spectrometer
tandem mass spectrometry
validation process
vegetable
Chromatography, High Pressure Liquid
Fruit
Insecticides
Mass Spectrometry
Organophosphorus Compounds
Reproducibility of Results
Vegetables
Brassica oleracea var. capitata
Vitaceae
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DOI
https://doi.org/10.1016/s0021-9673(03)01209-3
TNO identifier
237325
ISSN
0021-9673
Source
Journal of Chromatography A, 1015 (1-2), 119-127
Document type
article