Title
Genomic diversity within the enterobacter cloacae complex
Author
TNO Kwaliteit van Leven
Paauw, A.
Caspers, M.P.M.
Schuren, F.H.J.
Leverstein-van Hall, M.A.
Delétoile, A.
Montijn, R.C.
Verhoef, J.
Fluit, A.C.
Publication year
2008
Abstract
Background: Isolates of the Enterobacter cloacae complex have been increasingly isolated as nosocomial pathogens, but phenotypic identification of the E. cloacae complex is unreliable and irreproducible. Identification of species based on currently available genotyping tools is already superior to phenotypic identification, but the taxonomy of isolates belonging to this complex is cumbersome. Methodolgy/Principal Findings: This study shows that multilocus sequence analysis and comparative genomic hybridization based on a mixed genome array is a powerful method for studying species assignment within the E. cloacae complex. The E. cloacae complex is shown to be evolutionarily divided into two clades that are genetically distinct from each other. The younger first clade is genetically more homogenous, contains the Enterobacter hormaechei species and is the most frequently cultured Enterobacter species in hospitals. The second and older clade consists of several (sub)species that are genetically more heterogonous. Genetic markers were identified that could discriminate between the two clades and cluster 1. Conclusions/Significance: Based on genomic differences it is concluded that some previously defined (clonal and heterogenic) (sub)species of the E. cloacae complex have to be redefined because of disagreements with known or proposed nomenclature. However, further improved identification of the redefined species will be possible based on novel markers presented here. © 2008 Paauw et al. Chemicals / CAS: Bacterial Proteins; DNA, Bacterial
Subject
Healthy Living
Food and Nutrition
Cladistics
comparative genomic hybridization
Enterobacter hormaechei
microarray analysis
molecular evolution
multilocus sequence typing
species comparison
species identification
bacterial genome
DNA sequence
nucleic acid hybridization
phylogeny
Enterobacter
Enterobacter cloacae
Genetic Variation
Genome, Bacterial
Genotype
Nucleic Acid Hybridization
Phylogeny
Sequence Analysis, DNA
To reference this document use:
http://resolver.tudelft.nl/uuid:6ca22330-3d98-44cb-8dc7-7f0ed36448d4
DOI
https://doi.org/10.1371/journal.pone.0003018
TNO identifier
240960
ISSN
1932-6203
Source
PLoS ONE, 3 (3)
Document type
article