Title
Highly hydrolytic reuteransucrase from probiotic Lactobacillus reuteri strain ATCC 55730
Author
TNO Kwaliteit van Leven
Kralj, S.
Stripling, E.
Sanders, P.
van Geel-Schutten, G.H.
Dijkhuizen, L.
Publication year
2005
Abstract
Lactobacillus reuteri strain ATCC 55730 (LB BIO) was isolated as a pure culture from a Reuteri tablet purchased from the BioGaia company. This probiotic strain produces a soluble glucan (reuteran), in which the majority of the linkages are of the alpha-(1→4) glucosidic type (∼70%). This reuteran also contains alpha-(1→6)-linked glucosyl units and 4,6-disubstituted alpha-glucosyl units at the branching points. The LB BIO glucansucrase gene (gtfO) was cloned and expressed in Escherichia coli, and the GTFO enzyme was purified. The recombinant GTFO enzyme and the LB BIO culture supernatants synthesized identical glucan polymers with respect to linkage type and size distribution. GTFO thus is a reuteransucrase, responsible for synthesis of this reuteran polymer in LB BIO. The preference of GTFO for synthesizing alpha-(1→4) linkages is also evident from the oligosaccharides produced from sucrose with different acceptor substrates, e.g., isopanose from isomaltose. GTFO has a relatively high hydrolysis/transferase activity ratio. Complete conversion of 100 mM sucrose by GTFO nevertheless yielded large amounts of reuteran, although more than 50% of sucrose was converted into glucose. This is only the second example of the isolation and characterization of a reuteransucrase and its reuteran product, both found in different L. reuteri strains. GTFO synthesizes a reuteran with the highest amount of alpha-(1→4) linkages reported to date. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Subject
Food technology
Cell culture
Enzymes
Escherichia coli
Hydrolysis
Microbiology
Polymers
Polysaccharides
Size determination
Strain
Substrates
Sugar (sucrose)
BioGaia Company (CO)
Lactobacillus reuteri strain ATCC 55730
Probiotic strain
Reuteransucrase
Bacteria
Lactobacillus reuteri
Glucans
Glucosyltransferases
Kinetics
Lactobacillus
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Probiotics
Recombinant Proteins
Sequence Analysis, DNA
Escherichia coli
Lactobacillus reuteri
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DOI
https://doi.org/10.1128/aem.71.7.3942-3950.2005
TNO identifier
238587
ISSN
0099-2240
Source
Applied and Environmental Microbiology, 71 (71), 3942-3950
Document type
article