Print Email Facebook Twitter Developmental immunotoxicity of di-n-octyltin dichloride (DOTC) in an extended one-generation reproductive toxicity study Title Developmental immunotoxicity of di-n-octyltin dichloride (DOTC) in an extended one-generation reproductive toxicity study Author Tonk, E.C.M. de Groot, D.M.G. Penninks, A.H. Waalkens-Berendsen, I.D.H. Wolterbeek, A.P.M. Piersma, A.H. van Loveren, H. Publication year 2011 Abstract Developmental immunotoxicity assessment is considered ready for inclusion in developmental toxicity studies. Further evaluation of proposed and additional assays is needed to determine their utility in assessing developmental immunotoxicity. In this study, a wide range of immunological parameters was included in an extended one-generation reproductive toxicity protocol. F0 Wistar rats were exposed to DOTC via the feed (0, 3, 10, and 30mg/kg) during pre-mating, mating, gestation and lactation and subsequently F1 were exposed from weaning until sacrifice. Immune assessments by several immune parameters were performed at PNDs 21, 42 and 70. The T cell-dependent antibody response to Keyhole Limpet hemocyanin (KLH) was assessed following subcutaneous immunizations with KLH on PNDs 21 and 35 and the delayed-type hypersensitivity response (DTH) against KLH was evaluated at PND 49. No effects were found on PND 21. While effects on lymphocyte subpopulations in the thymus were only observed in the 30. mg/kg group on PND 42, effects on lymphocyte subpopulations in the spleen were found in the 30. mg/kg group on both PNDs 42 and 70. The DTH response already showed an effect at 3. mg/kg and was the overall critical endpoint. The results from this study support the inclusion of splenocyte subpopulation parameters in developmental toxicity studies and identified the DTH response as an important functional parameter. © 2011 Elsevier Ireland Ltd. Subject Triskelion BV LifeTAP - Toxicology and Applied Pharmacology QS - Quality & SafetyEELS - Earth, Environmental and Life SciencesNutritionDelayed-type hypersensitivity response (DTH)Developmental immunotoxicityExtended one-generation reproductive toxicity study (EOGRTS)Organotin To reference this document use: http://resolver.tudelft.nl/uuid:54f92d88-578d-47cd-82b4-1cb3c37c0a03 DOI https://doi.org/10.1016/j.toxlet.2011.04.027 TNO identifier 430090 ISSN 0378-4274 Source Toxicology Letters, 204 (2-3), 156-163 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.