Print Email Facebook Twitter Usefulness and limitations of Actilyse® for standardisation of tissue-type plasminogen activator (t-PA) assays in blood Title Usefulness and limitations of Actilyse® for standardisation of tissue-type plasminogen activator (t-PA) assays in blood Author Huisman, L.G.M. van Griensven, J.M.T. Kluft, C. TNO Preventie en Gezondheid Publication year 1996 Abstract Standardisation of t-PA antigen and activity assays requires a t-PA standard which ideally is a single preparation suitable for all assays. Most t-PA preparations, however, are not homogeneous; they can contain several forms of t-PA: single-chain and two-chain t-PA, and two glycoforms (I and II). In this study, the behaviour of Actilyse recombinant t-PA in several assays was studied and especially the effect of the two glycosylation forms on the efficiency of the assay. In two antigen assays and a direct assay on a chromogenic substrate we observed no specificity difference for different molecular forms of Actilyse whereas, in two-step assays based on plasminogen activation, such differences were present. It is concluded that for each assay the sensitivity to different forms of t-PA should be determined, in order to be able to decide whether or not Actilyse is a suitable standard preparation. Subject BiologyAlteplaseTissue plasminogen activatorAssayBlood levelConference paperControlled studyFibrinolysisHumanStandardization To reference this document use: http://resolver.tudelft.nl/uuid:45ea7515-0567-4639-9675-e00e91f96878 TNO identifier 233525 ISSN 0268-9499 Source Fibrinolysis, 10 (SUPPL. 2), 125-127 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.