Title
Radiosensitization of mammalian cells by diamide
Author
Vos, O.
Grant, G.A.
Budke, L.
Medisch Biologisch Laboratorium RVO-TNO
Publication year
1976
Abstract
The effect of diamide on the radiosensitivity of T cells was investigated under oxic and anoxic conditions. The compound was found to sensitize the cells under both conditions. Under oxic conditions, exposure for 10 min before and during irradiation to 0.1, 0.5 and 1.0 mM diamide produced dose modifying factors of 0.81, 0.60 and 0.55, respectively. Under anoxic conditions, exposure for 10 min before and during irradiation to 0.5 mM produced a dose modifying factor of 0.34. When the cells in oxic conditions were exposed for just 20 min before irradiation, the sensitizing effect was smaller, but some sensitization effect was still apparent after a 120 min interval between diamide treatment and irradiation. Diamide also sensitized the cells after irradiation, but this effect was less than when it was present during irradiation. The presence of whole rat blood in the incubation medium prevented sensitization. No sensitization could be detected in the whole animal. It is proposed that sensitization is due to lack of capacity for repair of radicals by hydrogen transfer and biochemical repair processes. Chemicals/CAS: chlorpropamide, 94-20-2; oxygen, 7782-44-7; penicillin G, 1406-05-9, 61-33-6; streptomycin, 57-92-1; Oxygen, 7782-44-7; Radiation-Sensitizing Agents
Subject
Biology
Chlorpropamide
Oxygen
Penicillin g
Streptomycin
Blood
Cell culture
Hypoxia
In vitro study
Mouse
Radiosensitization
Rat
T lymphocyte
Theoretical study
X ray
Animal
Blood
Cell Line
Cell Survival
Dose-Response Relationship, Drug
Dose-Response Relationship, Radiation
Male
Mice
Mice, Inbred CBA
Oxygen
Radiation-Sensitizing Agents
Rats
X-Rays
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TNO identifier
228091
ISSN
0955-3002
Source
International Journal of Radiation Biology, 29 (6), 513-522
Document type
article