Title
Quantitative analysis of O-isopropyl methylphosphonic acid in serum samples of Japanese citizens allegedly exposed to sarin: Estimation of internal dosage
Author
Noort, D.
Hulst, A.G.
Platenburg, D.H.J.M.
Polhuijs, M.
Benschop, H.P.
Prins Maurits Laboratorium TNO
Publication year
1998
Abstract
A convenient and rapid micro-anion exchange liquid chromatography (LC) tandem electrospray mass spectrometry (MS) procedure was developed for quantitative analysis in serum of O-isopropyl methylphosphonic acid (IMPA), the hydrolysis product of the nerve agent sarin. The mass spectrometric procedure involves negative or positive ion electrospray ionization and multiple reaction monitoring (MRM) detection. The method could be successfully applied to the analysis of serum samples from victims of the Tokyo subway attack and of an earlier incident at Matsumoto, Japan. IMPA levels ranging from 2 to 135 ng/ml were found. High levels of IMPA appear to correlate with low levels of residual butyrylcholinesterase activity in the samples and vice versa. Based on our analyses, the internal and exposure doses of the victims were estimated. In several cases, the doses appeared to be substantially higher than the assumed lethal doses in man.
Subject
Defence
Chemical warfare agents
LC tandem MS
Multiple reaction monitoring
O-isopropyl methylphosphonic acid
Sarin
Cholinesterase
Fluoride ion
Methylphosphonic acid
O isopropyl metylphosphonic acid
Sarin
Unclassified drug
Anion exchange chromatography
Article
Blood level
Chemical structure
Chemical warfare
Diagnostic value
Enzyme activity
Enzyme binding
Enzyme inhibition
Human
Hydrolysis
Japan
Lethal dose
Methodology
Population exposure
Priority journal
Tandem mass spectrometry
Toxicity testing
Butyrylcholinesterase
Chemistry, Analytical
Chromatography, Liquid
Environmental Exposure
Female
Humans
Male
Mass Spectrometry
Molecular Structure
Organophosphorus Compounds
Butyrylcholinesterase, EC 3.1.1.-
Isopropyl methylphosphonic acid, 1832-54-8
Organophosphorus Compounds
Sarin, 107-44-8
To reference this document use:
http://resolver.tudelft.nl/uuid:429efbb4-4cda-4516-beac-1d3ad54835b2
DOI
https://doi.org/10.1007/s002040050559
TNO identifier
234683
ISSN
0340-5761
Source
Archives of Toxicology, 72 (10), 671-675
Document type
article